Gould S J, Howard S
Department of Histopathology, University College, London, U.K.
J Pathol. 1988 Oct;156(2):129-35. doi: 10.1002/path.1711560207.
The characteristics of the germinal matrix vasculature were studied in the developing fetal brain using immunocytochemical methods. A preliminary comparative immunocytochemical study was made on six fetal brains to compare endothelial staining by Ulex europaeus I lectin with that of antibody to Factor VIII related antigen. Ulex was found to stain germinal layer vessels better than Factor VIII related antigen. Subsequently, the germinal layers of a further 15 fetal and preterm infant brains ranging from 13 to 35 weeks' gestation were stained with Ulex europaeus I to demonstrate the vasculature. With increasing gestation, there was a gradual increase in vessel density, particularly of capillaries. This was not a uniform process. A plexus of capillaries was prominent immediately beneath the ependyma while the more central parts of the germinal matrix contained fewer, but often larger diameter, vessels. The variation in vessel density which was a feature of the later gestation brains may have implications for local blood flow and may be a factor in haemorrhage at this site.
利用免疫细胞化学方法，对发育中的胎儿大脑生发基质血管系统的特征进行了研究。对六个胎儿大脑进行了初步的比较免疫细胞化学研究，以比较荆豆凝集素I（Ulex europaeus I lectin）对内皮细胞的染色与抗VIII因子相关抗原抗体的染色情况。结果发现，荆豆凝集素I对生发层血管的染色效果优于VIII因子相关抗原。随后，用荆豆凝集素I对另外15个孕龄在13至35周的胎儿和早产儿大脑的生发层进行染色，以显示血管系统。随着孕周的增加，血管密度逐渐增加，尤其是毛细血管。这并非一个均匀的过程。室管膜下方紧邻处有一个明显的毛细血管丛，而生发基质较中心的部分血管较少，但直径通常较大。孕周较大的胎儿大脑中血管密度的变化可能对局部血流有影响，并且可能是该部位出血的一个因素。
