Silva Miriam A, Santos Anna Raquel R, Rocha Leticia B, Caetano Bruna A, Mitsunari Thais, Santos Luanda I, Polatto Juliana M, Horton Denise S P Q, Guth Beatriz E C, Dos Santos Luís Fernando, Piazza Roxane M F
Laboratório de Bacteriologia, Instituto Butantan, São Paulo 05503-900, Brasil.
Departamento de Microbiologia, Imunologia, Parasitologia, Escola Paulista de Medicina Universidade Federal de São Paulo, São Paulo 04023-062, Brasil.
Microorganisms. 2019 Aug 21;7(9):276. doi: 10.3390/microorganisms7090276.
Shiga toxin (Stx)-producing (STEC) and its subgroup enterohemorrhagic are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx and Stx toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx plus mAb stx) showed 99% sensitivity and 97% specificity. Individually, Stx antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections.
产志贺毒素（Stx）的大肠杆菌（STEC）及其亚群肠出血性大肠杆菌是引起腹泻的重要病原体，腹泻可能并发出血性结肠炎和溶血尿毒综合征，后者是儿童急性肾衰竭的主要原因。早期诊断对临床治疗至关重要，因为不建议对STEC感染进行抗生素治疗。先前获得的针对Stx和Stx毒素的抗体被用于评估乳胶凝集试验（LAT）、侧向流动分析（LFA）和捕获酶联免疫吸附测定（cEIA）检测STEC的敏感性和特异性。LAT（单克隆抗体Stx加单克隆抗体stx）显示出99%的敏感性和97%的特异性。单独来看，在cEIA和LFA检测中，Stx抗体的敏感性分别为95.5%和94%，特异性分别为97%和99%。在两种检测中，Stx抗体的敏感性均为92%，在cEIA和LFA检测中的特异性分别为100%和98%。这些结果使我们能够得出结论，我们拥有用于诊断STEC感染的可靠工具。
