Jiang Xiaofeng, Wang Xiaoyu, Yao Chuang, Zhu Shuxian, Liu Lu, Liu Ronghua, Li Lidong
State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
Key Laboratory of Extraordinary Bond Engineering and Advance Materials Technology (EBEAM) of Chongqing, Yangtze Normal University, Chongqing 408100, People's Republic of China.
J Phys Chem Lett. 2019 Sep 5;10(17):5237-5243. doi: 10.1021/acs.jpclett.9b02046. Epub 2019 Aug 26.
Here, we develop bifunctional ligand-engineered gold nanoclusters (AuNCs) as signal amplifying reporters for multimode imaging. Modified streptavidin (SA) and biotin alkyl acid-based ligands were applied to AuNCs to form AuNC-SA and AuNC-biotin. The zwitterionic ligands promoted bioassembly and avoided nonspecific adsorption. The AuNCs resisted aggregation-induced quenching and showed strong emission benefited from biological self-assembly. The engineered AuNCs featured stable emission, a large two-photon absorption cross section, long fluorescence lifetime, and good biocompatibility. Thus, cell-expressed antigen-induced protein-binding events were effectively converted into signals from the biological assemble of AuNCs. We performed a comprehensive assay of specific antigens and the cell structure, through one-photon imaging, two-photon imaging, and fluorescence lifetime imaging of AuNCs in a simple, sensitive, and reliable way.
在此，我们开发了双功能配体工程化金纳米簇（AuNCs）作为用于多模态成像的信号放大报告分子。将修饰的链霉亲和素（SA）和基于生物素烷基酸的配体应用于AuNCs，以形成AuNC-SA和AuNC-生物素。两性离子配体促进生物组装并避免非特异性吸附。AuNCs抵抗聚集诱导的猝灭，并由于生物自组装而表现出强发射。工程化的AuNCs具有稳定的发射、大的双光子吸收截面、长荧光寿命和良好的生物相容性。因此，细胞表达的抗原诱导的蛋白质结合事件被有效地转化为来自AuNCs生物组装的信号。我们通过AuNCs的单光子成像、双光子成像和荧光寿命成像，以简单、灵敏和可靠的方式对特定抗原和细胞结构进行了全面分析。
