Furness P N, Turner D R
Department of Pathology, University Hospital, Nottingham, United Kingdom.
Virchows Arch A Pathol Anat Histopathol. 1988;413(6):551-3. doi: 10.1007/BF00750396.
Chronic serum sickness glomerulonephritis was induced in 20 Wistar rats, using radiolabelled, chemically cationized bovine serum albumin (BSA) as antigen. Four days after the last injection of antigen, when relocation of antigen within the rat had effectively ceased, the rats were given a single large intraperitoneal dose of either non-immune rat gamma globulin or anti-BSA rat gamma globulin. Ten days later the rats were killed. The rats which had received the anti-BSA globulin had significantly more antigen in renal cortex and in isolated glomeruli than the control group. They also had larger mesangial deposits as assessed by morphometry at electron microscope level; assessment of subepithelial deposits provided equivocal results. These findings provide direct confirmation that circulating antibody which is directed against an antigen which is trapped within deposits in the glomerulus will inhibit the removal of the antigen and deposits from the mesangium.
使用放射性标记的化学阳离子化牛血清白蛋白（BSA）作为抗原，在20只Wistar大鼠中诱发慢性血清病肾小球肾炎。在最后一次注射抗原4天后，当抗原在大鼠体内的重新分布有效停止时，给大鼠单次腹腔内注射大剂量的非免疫大鼠γ球蛋白或抗BSA大鼠γ球蛋白。10天后处死大鼠。接受抗BSA球蛋白的大鼠肾皮质和分离的肾小球中的抗原明显多于对照组。通过电子显微镜水平的形态计量学评估，它们的系膜沉积物也更大；上皮下沉积物的评估结果不明确。这些发现直接证实，针对被困在肾小球沉积物中的抗原的循环抗体将抑制系膜中抗原和沉积物的清除。
