清除含有过量未改变或阳离子抗原的上皮下免疫复合物。

Removal of subepithelial immune complexes with excess unaltered or cationic antigen.

作者信息

Agodoa L Y, Mannik M

出版信息

Kidney Int. 1987 Jul;32(1):13-8. doi: 10.1038/ki.1987.165.

DOI:10.1038/ki.1987.165

Abstract

Since cationic molecules are known to pass through the lamina densa of the glomerular basement membrane at higher rates than anionic molecules, the ability of cationic antigen to disperse subepithelial glomerular immune deposits was investigated in rats. Subepithelial glomerular immune deposits were formed in rats by the sequential administration of cationized human serum albumin (HSAED) and purified rabbit antibodies to human serum albumin (HSA). In vitro the addition of fifty-fold excess HSA or HSAED to immune precipitates formed with HSAED and antibodies to human serum albumin (antiHSA) solubilized the precipitates to comparable degree. Excess HSA or HSAED was given intravenously to rats which already had HSAED-antiHSA immune deposits in glomeruli. Serial renal biopsies were obtained and examined. Control animals received saline or nonspecific cationic molecules, protamine sulfate or cationized rabbit serum albumin, without any effect on the persistence of immune deposits in glomeruli. The injection of 10 mg of HSAED caused complete disappearance of glomerular immune deposits by 48 hours. In contrast, 100 mg of HSA was required to achieve the same effect. Thus, cationic antigens are more efficient than anionic antigens in the removal of subepithelial glomerular immune deposits.

摘要

由于已知阳离子分子穿过肾小球基底膜致密层的速率高于阴离子分子，因此在大鼠中研究了阳离子抗原分散上皮下肾小球免疫沉积物的能力。通过依次给予阳离子化人血清白蛋白（HSAED）和纯化的兔抗人血清白蛋白（HSA）抗体，在大鼠中形成上皮下肾小球免疫沉积物。在体外，向用HSAED和抗人血清白蛋白抗体（抗HSA）形成的免疫沉淀物中加入五十倍过量的HSA或HSAED，可使沉淀物溶解至相当程度。将过量的HSA或HSAED静脉注射给肾小球中已经存在HSAED-抗HSA免疫沉积物的大鼠。获取并检查系列肾活检标本。对照动物接受盐水或非特异性阳离子分子、硫酸鱼精蛋白或阳离子化兔血清白蛋白，对肾小球中免疫沉积物的持续存在没有任何影响。注射10mg HSAED可使肾小球免疫沉积物在48小时内完全消失。相比之下，需要100mg HSA才能达到相同效果。因此，在清除上皮下肾小球免疫沉积物方面，阳离子抗原比阴离子抗原更有效。