褪黑素处理在冷藏过程中保持石榴果实的营养特性。
Melatonin treatment maintains nutraceutical properties of pomegranate fruits during cold storage.
机构信息
Department of Horticultural Science, Imam Khomeini International University, Qazvin, Iran.
Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
出版信息
Food Chem. 2020 Jan 15;303:125385. doi: 10.1016/j.foodchem.2019.125385. Epub 2019 Aug 19.
In this study, the mechanism activated by melatonin treatment at 100 µM for maintaining nutraceutical properties in pomegranate fruits during storage at 4 °C for 120 days was investigated. Our results showed that the higher G6PDH and 6PGDH activities in pomegranate fruits treated with melatonin may be responsible for sufficient supply of intracellular NADPH. Also, higher AA and GSH accumulation in pomegranate fruits treated with melatonin may ascribe to higher APX and GR activities coincided with lower AAO activity. In addition, pomegranate fruits treated with melatonin exhibited significantly higher PAL activity resulting in higher phenols and anthocyanins accumulation as well as higher DPPH scavenging capacity. Additionally, higher AOX gene expression in pomegranate fruits treated with melatonin may be beneficial for ROS scavenging molecules accumulation. Therefore, maintaining nutraceutical properties of pomegranate fruits treated with melatonin may ascribe to sufficient intracellular NADPH supply by promoting G6PDH and 6PGDH activities during cold storage.
本研究旨在探讨 100µM 褪黑素处理在维持石榴果实营养特性方面的作用机制，该处理可在 4°C 下贮藏 120 天。研究结果表明，褪黑素处理可提高石榴果实中的 G6PDH 和 6PGDH 活性，从而为细胞内 NADPH 的充足供应提供保障。此外，褪黑素处理还可提高石榴果实中的 AA 和 GSH 积累，这与 APX 和 GR 活性的升高以及 AAO 活性的降低有关。另外，褪黑素处理还可使 PAL 活性显著升高，从而导致酚类物质和花青素的积累增加，以及 DPPH 清除能力增强。此外，褪黑素处理的石榴果实中的 AOX 基因表达水平升高，这有利于 ROS 清除分子的积累。因此，褪黑素处理可通过促进 G6PDH 和 6PGDH 活性来维持石榴果实的营养特性，从而在冷藏过程中为细胞内 NADPH 的供应提供保障。
Zotero 插件