Bai Shiping, Zhang Keying, Ding Xuemei, Wang Jianping, Zeng Qiufeng, Peng Huanwei, Bai Jie, Xuan Yue, Su Zuowei, Wu Bin
Institute of Animal Nutrition, Sichuan Agricultural University, Huimin Road 211#, Wenjiang District, Chengdu 611130, Sichuan, China.
Key Laboratory of Animal Disease-resistant Nutrition, Ministry of Education, Sichuan Agricultural University, Huimin Road 211#, Wenjiang District, Chengdu 611130, Sichuan, China.
Animals (Basel). 2019 Aug 15;9(8):559. doi: 10.3390/ani9080559.
Organic manganese (Mn) sources can replace inorganic Mn as dietary Mn supplements in poultry. To compare the uptake of Mn from the Mn-lysine complex (MnLys) and MnSO, we first established the primary chicken intestinal epithelial cells (IECs) model and used it to determine Mn uptake. The MnLys increased the uptake of Mn compared to MnSO. The uptake of Mn decreased in the IECs with Fe addition in the medium regardless of the Mn sources. The MnLys decreased the Mn efflux transporter ferroportin 1 (FPN1) mRNA level but did not influence the Mn influx transporter divalent metal transporter 1 (DMT1) mRNA expression when compared to MnSO. The results above indicated that the increase of Mn accumulation for MnLys at least partly was due to the decrease of Mn efflux by reduced FPN1 expression. The addition of -ethylmaleimide, an L-lysine transport system y inhibitor, decreased the uptake of Mn from MnLys but did not affect the uptake of Mn from MnSO. The cycloheximide, as an L-lysine transport system b activator, increased the uptake of Mn from MnLys, whereas they did not influence the uptake of Mn from MnSO. The MnLys increased the system y members cationic amino acid transporter (CAT) 1 and CAT2, and system b components rBAT and bAT mRNA expression when compared to MnSO. These results suggested that the uptake of MnLys complex might be transported by CAT1/2 and system b, which was different from the ionized Mn uptake pathway. In conclusion, the uptake of Mn from MnLys complex not only might be uptake through the ionized Mn pathway, but also appeared to be transported through the CAT1/2 and system b in primary chicken IECs.
有机锰（Mn）源可替代无机锰作为家禽日粮中的锰补充剂。为比较锰赖氨酸络合物（MnLys）和硫酸锰（MnSO₄）中锰的吸收情况，我们首先建立了原代鸡肠上皮细胞（IECs）模型，并用于测定锰的吸收。与MnSO₄相比，MnLys增加了锰的吸收。无论锰源如何，培养基中添加铁后IECs中锰的吸收均降低。与MnSO₄相比，MnLys降低了锰外流转运蛋白铁转运蛋白1（FPN1）的mRNA水平，但不影响锰内流转运蛋白二价金属转运蛋白1（DMT1）的mRNA表达。上述结果表明，MnLys的锰积累增加至少部分是由于FPN1表达降低导致锰外流减少。添加L-赖氨酸转运系统y抑制剂N-乙基马来酰亚胺可降低MnLys中锰的吸收，但不影响MnSO₄中锰的吸收。作为L-赖氨酸转运系统b激活剂的环己酰亚胺增加了MnLys中锰的吸收，而它们不影响MnSO₄中锰的吸收。与MnSO₄相比，MnLys增加了系统y成员阳离子氨基酸转运蛋白（CAT）1和CAT2以及系统b成分rBAT和b⁰⁺AT的mRNA表达。这些结果表明，MnLys络合物的吸收可能通过CAT1/2和系统b转运，这与离子化锰的吸收途径不同。总之，原代鸡IECs中MnLys络合物的锰吸收不仅可能通过离子化锰途径吸收，而且似乎还通过CAT1/2和系统b转运。
