Hadži San, Bončina Matjaž, Lah Jurij
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia.
Methods Mol Biol. 2019;2035:117-130. doi: 10.1007/978-1-4939-9666-7_7.
Guanine-rich DNA oligonucleotides can adopt G-quadruplex (G4) structures in the presence of specific cations. Folding and unfolding of G4 can be characterized thermodynamically, providing the information on the stability of various G4 conformations. We show how the relevant thermodynamic and sometimes kinetic parameters are obtained by employing differential scanning calorimetry (DSC) and global fitting of an appropriate model to the DSC data. We demonstrate that best-fit values of the thermodynamic parameters can be interpreted in terms of the driving forces accompanying the G4 folding/interconversion and how they are translated into the phase diagrams, which provide an elegant description of the G4 phase space over a wide range of solution conditions.
富含鸟嘌呤的DNA寡核苷酸在特定阳离子存在的情况下可以形成G-四链体（G4）结构。G4的折叠和解折叠可以通过热力学进行表征，从而提供有关各种G4构象稳定性的信息。我们展示了如何通过差示扫描量热法（DSC）以及将适当模型对DSC数据进行全局拟合来获得相关的热力学参数，有时还能获得动力学参数。我们证明，热力学参数的最佳拟合值可以根据伴随G4折叠/相互转换的驱动力来解释，以及它们如何转化为相图，相图在广泛的溶液条件下对G4相空间进行了精妙的描述。
