Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
Biol Trace Elem Res. 2020 Jun;195(2):579-590. doi: 10.1007/s12011-019-01872-7. Epub 2019 Aug 23.
The present study aims to evaluate the efficacy of selenium (Se) alone or combined with carbamazepine (CBZ) against the adverse effects induced by the chemoconvulsant pentylenetetrazole (PTZ) in the cortex of adult male rats. Electrocorticogram (ECoG) and oxidative stress markers were implemented to evaluate the differences between treated and untreated animals. Animals were divided into five groups: control group that received i.p. saline injection, PTZ-treated group that received a single i.p. injection of PTZ (60 mg/kg) for induction of seizures followed by a daily i.p. injection of saline, Se-treated group that received an i.p. injection of sodium selenite (0.3 mg/kg/day) after PTZ administration, CBZ-treated group that received orally CBZ (80 mg/kg/day) after PTZ administration, and combination (Se plus CBZ)-treated group that received an oral administration of CBZ (80 mg/kg/day) followed by an i.p. injection of sodium selenite (0.3 mg/kg/day) after PTZ administration. Quantitative analyses of the ECoG indices and the neurochemical parameters revealed that Se and CBZ have mitigated the adverse effects induced by PTZ. The main results were decrease in the number of epileptic spikes, restoring the normal distribution of slow and fast ECoG frequencies and attenuation of most of the oxidative stress markers. However, there was an increase in lipid perioxidation marker in combined treatment of CBZ and Se. The electrophysiological and neurochemical data proved the potential of these techniques in evaluating the treatment's efficiency and suggest that supplementation of Se with antiepileptic drugs (AEDs) may be beneficial in ameliorating most of the alterations induced in the brain as a result of seizure insults and could be recommended as an adjunct therapy with AEDs.
本研究旨在评估单独使用硒（Se）或与卡马西平（CBZ）联合使用对戊四氮（PTZ）致痫剂诱导的成年雄性大鼠皮质不良反应的疗效。采用脑电图（ECoG）和氧化应激标志物来评估处理组与未处理组之间的差异。动物被分为五组：对照组接受腹腔注射生理盐水，PTZ 处理组接受单次腹腔注射 PTZ（60mg/kg）以诱导癫痫发作，然后每天腹腔注射生理盐水，Se 处理组在给予 PTZ 后接受腹腔注射亚硒酸钠（0.3mg/kg/天），CBZ 处理组在给予 PTZ 后口服卡马西平（80mg/kg/天），联合（Se 加 CBZ）处理组在给予 PTZ 后口服卡马西平（80mg/kg/天），然后腹腔注射亚硒酸钠（0.3mg/kg/天）。ECoG 指数和神经化学参数的定量分析表明，Se 和 CBZ 减轻了 PTZ 引起的不良反应。主要结果是癫痫棘波数量减少，恢复了慢波和快波 ECoG 频率的正常分布，并减轻了大多数氧化应激标志物。然而，在 CBZ 和 Se 的联合治疗中，脂质过氧化标志物增加。电生理和神经化学数据证明了这些技术在评估治疗效果方面的潜力，并表明补充 Se 与抗癫痫药物（AEDs）联合使用可能有助于改善因癫痫发作引起的大脑大部分改变，并可作为 AEDs 的辅助治疗推荐。
