Klinke Sebastián, Rinaldi Jimena, Goldbaum Fernando A, Suarez Sebastián, Otero Lisandro H
Fundación Instituto Leloir, IIBBA-CONICET, Av. Patricias Argentinas 435, C1405BWE, Buenos Aires, Argentina.
Plataforma Argentina de Biología Estructural y Metabolómica PLABEM, Av. Patricias Argentinas 435, C1405BWE, Buenos Aires, Argentina.
Biochem Mol Biol Educ. 2019 Nov;47(6):700-707. doi: 10.1002/bmb.21296. Epub 2019 Aug 24.
X-ray crystallography provides structural information of molecules at the atomic level, being a central technique at the forefront of science and technology. However, crystallography teaching is not usually implemented in biochemistry lab classes due to its complex execution by nonexpert users. Here, we report the basic step-by-step workflow performed by crystallographers in order to solve the three-dimensional structure of a protein. All these activities were executed in a course for Latin-American graduate students with no previous knowledge on X-ray crystallography entitled "Crystallography in Structural Biology: why do we need a protein crystal, and how do we get it?." We would like to share our experience with the educational research community, with the main purpose being to enrich teaching in biochemistry and structural molecular biology by performing a series of interesting laboratory and computer experiments. © 2019 International Union of Biochemistry and Molecular Biology, 47(6):700-707, 2019.
X射线晶体学能够在原子水平上提供分子的结构信息，是处于科学技术前沿的核心技术。然而，由于非专业人员执行起来较为复杂，晶体学教学通常不在生物化学实验课中开展。在此，我们报告晶体学家为解析蛋白质三维结构所执行的基本分步工作流程。所有这些活动均在一门面向拉丁美洲研究生的课程中进行，这些学生此前没有X射线晶体学方面的知识，该课程名为“结构生物学中的晶体学：我们为何需要蛋白质晶体，以及如何获得它？”。我们希望与教育研究界分享我们的经验，主要目的是通过开展一系列有趣的实验室和计算机实验，丰富生物化学和结构分子生物学的教学内容。© 2019国际生物化学与分子生物学联盟，47(6):700 - 707, 2019。
