School of Chemistry and Chemical Engineering, Southwest University. Chongqing, China; Analytical Chemistry Section, Faculty of Chemistry, University of Barcelona, Barcelona, Spain.
Analytical Chemistry Section, Faculty of Chemistry, University of Barcelona, Barcelona, Spain.
J Chromatogr A. 2020 Jan 4;1609:460459. doi: 10.1016/j.chroma.2019.460459. Epub 2019 Aug 14.
Sulfate (SO-sug) and sulfonate (SO-sug) arsenosugar standard solutions were obtained using preparative liquid chromatography. Several commercial algae samples were characterized (total contents and speciation) to select the most appropriate in relation to their arsenosugar contents. Water extracts from the selected sample (Fucus vesiculosus) were fractionated using a Hamilton PRP-X100 preparative column, and the presence of arsenic species in the isolated fractions was ascertained by IC-ICP-MS. Two of the fractions successfully presented only one arsenic species corresponding to sulfate and sulfonate arsenosugars at suitable concentrations. To unequivocally confirm the presence of both compounds, high-resolution mass spectrometry (ESI-TOF/MS) was used and the exact mass determined with errors lower than 0.5 ppm. The standard solutions obtained were successfully used to identify and quantify SO-sug and SO-sug in several edible algae samples purchased in local market. Total arsenic content for analyzed samples ranged from 34 to 57 mg kg, concentration values found for SO-sug ranged from 5 to 36 mg As kg and SO-sug was only found in fucus with a concentration of 9.3 mg As kg.
采用制备液相色谱法获得了硫酸盐（SO-sug）和磺酸盐（SO-sug）砷糖标准溶液。对几种商业藻类样品进行了特征分析（总含量和形态），以选择最适合其砷糖含量的样品。从选定的样品（泡叶藻）的水提取物中使用 Hamilton PRP-X100 制备柱进行分离，通过 IC-ICP-MS 确定分离馏分中砷形态的存在。两个馏分成功地仅呈现出一种砷形态，对应于硫酸盐和磺酸盐砷糖，浓度合适。为了明确确认这两种化合物的存在，使用了高分辨率质谱（ESI-TOF/MS），并确定了精确质量，误差低于 0.5ppm。获得的标准溶液成功地用于鉴定和定量当地市场购买的几种食用藻类样品中的 SO-sug 和 SO-sug。分析样品的总砷含量范围为 34 至 57mgkg，发现的 SO-sug 浓度范围为 5 至 36mgAskg，仅在褐藻中发现 SO-sug，浓度为 9.3mgAskg。
