不同锌浓度对活髓治疗部分稳定水泥的影响。
Effect of different zinc concentrations on partially-stabilized cement for vital pulp therapy.
机构信息
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
出版信息
J Formos Med Assoc. 2019 Dec;118(12):1610-1615. doi: 10.1016/j.jfma.2019.08.005. Epub 2019 Aug 21.
BACKGROUND/PURPOSE: We have developed and investigated the partially-stabilized cements (PSC) with Zn for vital pulp therapy due to their short setting time and high cell biocompatibility. However, the effect of PSC with different concentrations of Zn on setting time and biocompatibility remained unknown. Therefore, the purpose of this study was to determine the optimal concentration of Zn to be synthesized with PSC for vital pulp therapy.
METHODS
PSC with different weight percentages of Zn (5%, 7%, 10%) were synthesized to attain 5%Zn-PSC, 7%Zn-PSC, and 10%Zn-PSC. The initial and final setting times were measured using the Gillmore needles method, and the compressive strength tests were conducted using a universal testing machine. The phases of Zn-PSC powders were observed using an X-ray diffractometer (XRD). Human dental pulp stem cells (hDPSCs) were used to evaluate the biocompatibility and cytotoxicity of the materials via Alamar blue and LDH assays. Mineral trioxide aggregate (MTA) was used to be compared with Zn-PSC samples.
RESULTS
The initial and final setting times of PSC with different concentrations of Zn were reduced considerably compared to those of MTA. The results also indicated that the initial and final setting times decreased as the weight % of Zn increased. 5%Zn-PSC had the highest compressive strength among all tested materials. 5%Zn-PSC samples also displayed comparatively higher cell biocompatibility than 7% and 10% Zn-PSC samples. However, there was no significant difference between the 5%Zn-PSC and MTA in cell biocompatibility. In addition, the results of the LDH release assay indicated a low level of cytotoxicity among all the test samples.
CONCLUSION
5%Zn-PSC has a shorter setting time, better mechanical properties, and good biocompatibility and thus it has great potential for vital pulp therapy.
背景/目的：我们已经开发并研究了含有 Zn 的部分稳定化水泥（PSC），因为它具有较短的凝固时间和较高的细胞生物相容性，可用于活髓治疗。然而，含有不同浓度 Zn 的 PSC 对凝固时间和生物相容性的影响尚不清楚。因此，本研究的目的是确定用于活髓治疗的 PSC 中 Zn 的最佳合成浓度。
方法
合成了不同重量百分比 Zn（5%、7%、10%）的 PSC，以获得 5%Zn-PSC、7%Zn-PSC 和 10%Zn-PSC。使用 Gillmore 针法测量初始和最终凝固时间，并使用万能试验机进行压缩强度测试。使用 X 射线衍射仪（XRD）观察 Zn-PSC 粉末的相。用人牙髓干细胞（hDPSCs）通过 Alamar blue 和 LDH 测定法评估材料的生物相容性和细胞毒性。将矿物三氧化物聚合体（MTA）与 Zn-PSC 样品进行比较。
结果
与 MTA 相比，不同浓度 Zn 的 PSC 的初始和最终凝固时间大大缩短。结果还表明，随着 Zn 重量百分比的增加，初始和最终凝固时间减少。所有测试材料中，5%Zn-PSC 的抗压强度最高。5%Zn-PSC 样品的细胞生物相容性也比 7%和 10%Zn-PSC 样品高。然而，5%Zn-PSC 和 MTA 在细胞生物相容性方面没有显著差异。此外，LDH 释放测定的结果表明所有测试样品的细胞毒性水平较低。
结论
5%Zn-PSC 具有较短的凝固时间、更好的机械性能和良好的生物相容性，因此在活髓治疗中具有很大的潜力。
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