Slotkin T A, Seidler F J, Haim K, Cameron A M, Antolick L, Lau C
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina.
J Pharmacol Exp Ther. 1988 Dec;247(3):975-82.
Peripheral sympathetic neurons are thought to provide trophic regulatory signals for development of adrenergic target tissues. In the current study, we destroyed central catecholaminergic pathways in the neonatal rat by intracisternal administration of 6-hydroxydopamine, which compromises sympathetic tone without ablating peripheral nerve terminals. Measurements of norepinephrine levels and turnover confirmed the effectiveness of the treatment in projections to heart, lung and kidney. The impairment of sympathetic tone was associated with a deficit in cardiac beta adrenergic receptor binding capabilities; in contrast, binding sites in the lung were unaffected and renal receptors were up-regulated. Similarly, intracisternal administration of 6-hydroxydopamine produced tissue-selective alterations in ornithine decarboxylase activity and levels of the polyamines. These results support the view that neural activity exerts an influence on the biochemical development of sympathetic target tissues; however, other trophic factors may derive from the presence of intact nerve terminals themselves, as distinct from activity.
外周交感神经元被认为可为肾上腺素能靶组织的发育提供营养调节信号。在本研究中，我们通过脑池内注射6-羟基多巴胺破坏新生大鼠的中枢儿茶酚胺能通路，这会损害交感神经张力但不切除外周神经末梢。去甲肾上腺素水平和周转率的测量证实了该治疗对心脏、肺和肾脏投射的有效性。交感神经张力的损害与心脏β肾上腺素能受体结合能力的缺陷有关；相比之下，肺中的结合位点未受影响，而肾受体上调。同样，脑池内注射6-羟基多巴胺会导致鸟氨酸脱羧酶活性和多胺水平出现组织选择性改变。这些结果支持了神经活动对交感靶组织生化发育有影响的观点；然而，其他营养因子可能源于完整神经末梢本身的存在，与活动无关。
