Departamento de Bioquímica e Biologia Molecular e Programa de Pós-graduação Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Brazil.
Departamento de Ciências da Saúde, Laboratório de Microbiologia CEMICRO, UFSM, Brazil.
Comp Immunol Microbiol Infect Dis. 2019 Oct;66:101343. doi: 10.1016/j.cimid.2019.101343. Epub 2019 Aug 9.
Invertebrates have been instrumental in understanding the mechanisms involved in infectious diseases, considering the idea to replace, reduce and refine the use of mammals as well as to understand the basic principles of immune response in insect. We evaluated the consequences of Staphylococcus aureus-induced sepsis in the last instar nymphs of Nauphoeta cinerea injected with different concentrations of bacteria preserved in two culture media. Infected groups had a decrease in hemolymph metabolites (glucose, amino acids, total proteins, and cholesterol), in contrast to the proteins in the fat body. Higher concentrations of S. aureus caused permanent morphological alterations in adults, decrease in food consumption, increase in isolation, and increase in CFU until death of the cockroaches. Survival and protection of nymphs against a repeated and stronger challenge with the same bacteria varied according to the medium they were conserved. N. cinerea proves to be a suitable and promising model for studies related to bacterial infections.
无脊椎动物在理解传染病涉及的机制方面一直发挥着重要作用，因为考虑到用哺乳动物替代、减少和优化的使用，以及理解昆虫免疫反应的基本原理。我们评估了在不同浓度保存在两种培养基中的细菌注射到最后龄若虫体内诱导的金黄色葡萄球菌性败血症的后果。与脂肪体中的蛋白质相比，感染组的血淋巴代谢物（葡萄糖、氨基酸、总蛋白质和胆固醇）减少。较高浓度的金黄色葡萄球菌导致成虫出现永久性形态改变，食物消耗减少，隔离增加，CFU 增加，直至蟑螂死亡。若虫对同一细菌的重复和更强挑战的存活和保护因它们保存在不同的培养基而异。Nauphoeta cinerea 被证明是一个合适且有前途的细菌感染相关研究模型。
