Interdisciplinary Program of Marine-Bio, Electrical & Mechanical Engineering, Pukyong National University, Busan, South Korea.
Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, 48513, South Korea.
Lasers Med Sci. 2020 Apr;35(3):677-685. doi: 10.1007/s10103-019-02869-3. Epub 2019 Aug 24.
Primary hepatocellular carcinoma (HCC) and colon carcinoma are two of the most common clinical malignancies along with high morbidity and mortality. As low-power laser irradiation (LPLI) can induce cytotoxicity or cell apoptosis on several types of hyperplasia, LPLI may be a potential alternative treatment for gastroenterological cancers. The current in vitro study focused on LPLI-induced apoptosis and mechanism after 532-nm laser irradiation on two different carcinoma cells. Squamous cell carcinoma (VX2) and murine colon carcinoma (CT26) cells were cultured to test the feasibility of LPLI. The applied fluence varied from 0 to 600 J/cm. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide analysis, fluorescence imaging, wound healing assay, and cell apoptosis tests were performed 24 h post-irradiation to monitor cellular responses. The current results demonstrated a dose-dependent stimulatory effect of LPLI on the cell viability, migration, and apoptosis of VX2 and CT26 cells. The therapeutic fluence of 600 J/cm induced statistically significant inhibition in cell viability. Both the wound healing assay and the cell apoptosis tests confirmed that LPLI with high fluences could inhibit cell migration as well as induce cell apoptosis. The current findings demonstrate that LPLI might be a potential treatment for the carcinoma cells. Further studies will be performed to evaluate the feasibility of LPLI in in vivo tumor models.
原发性肝细胞癌（HCC）和结肠癌是最常见的两种临床恶性肿瘤，发病率和死亡率都很高。由于低功率激光照射（LPLI）可以诱导多种类型的增生细胞发生细胞毒性或细胞凋亡，因此 LPLI 可能是治疗胃肠道癌症的一种潜在替代方法。本体外研究聚焦于 532nm 激光照射两种不同癌细胞后 LPLI 诱导的细胞凋亡及其机制。培养鳞状细胞癌（VX2）和鼠结肠癌（CT26）细胞以测试 LPLI 的可行性。应用的剂量从 0 到 600J/cm 不等。照射后 24 小时进行 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴盐分析、荧光成像、划痕愈合试验和细胞凋亡试验，以监测细胞反应。目前的结果表明，LPLI 对 VX2 和 CT26 细胞的活力、迁移和凋亡具有剂量依赖性的刺激作用。600J/cm 的治疗剂量可显著抑制细胞活力。划痕愈合试验和细胞凋亡试验均证实，高剂量的 LPLI 可抑制细胞迁移并诱导细胞凋亡。目前的研究结果表明，LPLI 可能是治疗癌细胞的一种潜在方法。进一步的研究将评估 LPLI 在体内肿瘤模型中的可行性。
