Zhao Jianqiang, Wang Changmei, Liu Shuai, Su Xinyou, Ouyang Aimei
Department of Oncology, Jinan Central Hospital, Jinan City, Shandong Province 250012, People's Republic of China.
Onco Targets Ther. 2019 Aug 2;12:6175-6180. doi: 10.2147/OTT.S207079. eCollection 2019.
The present study aims to investigate the involvement of lncRNA GAPLINC in non-small lung cancer (NSCLC). The study included 70 patients with NSCLC (39 males and 31 females, 33 to 68 years, 49.3 ± 6.4 years). RT-qPCR, transient cell transfections, measurement of in vitro cell migration and invasion abilities and western blot were carrying out during the research. We showed that GAPLINC was up-regulated in NSCLC tissues and positively correlated with TGF-β1. In vitro cell experiment showed that over-expression of TGF-β1 significantly up-regulated the expression of GAPLINC, while over-expression of GAPLINC failed to affect TGF-β1. Follow-up study showed that high GAPLINC level in NSCLC tissue was closely correlated with poor survival rate of NSCLC patients. Over-expressions of TGF-β1 and GAPLINC resulted to accelerated migration and invasion of NSCLC cells. In addition, the silencing of GAPLINC siRNA attenuated the effect of TGF-β1 treatment. TGF-β1 may mediate lncRNA GAPLINC expression to promote NSCLC cell invasion and migration.
本研究旨在探究长链非编码RNA GAPLINC在非小细胞肺癌（NSCLC）中的作用。该研究纳入了70例NSCLC患者（39例男性和31例女性，年龄33至68岁，平均49.3±6.4岁）。研究过程中进行了逆转录定量聚合酶链反应（RT-qPCR）、瞬时细胞转染、体外细胞迁移和侵袭能力检测以及蛋白质免疫印迹法。我们发现，GAPLINC在NSCLC组织中表达上调，且与转化生长因子-β1（TGF-β1）呈正相关。体外细胞实验表明，TGF-β1过表达显著上调GAPLINC的表达，而GAPLINC过表达则未影响TGF-β1。随访研究显示，NSCLC组织中GAPLINC水平高与NSCLC患者生存率低密切相关。TGF-β1和GAPLINC过表达导致NSCLC细胞迁移和侵袭加速。此外，GAPLINC小干扰RNA（siRNA）沉默减弱了TGF-β1处理的效果。TGF-β1可能通过介导长链非编码RNA GAPLINC的表达来促进NSCLC细胞的侵袭和迁移。
