在哺乳动物细胞的细胞膜和胞质溶胶中对G蛋白GZ的α亚基进行免疫化学检测。
Immunochemical detection of the alpha-subunit of the G-protein, GZ, in membranes and cytosols of mammalian cells.
作者信息
Spicher K, Hinsch K D, Gausepohl H, Frank R, Rosenthal W, Schultz G
机构信息
Institut für Pharmakologie, Freie Universität Berlin, FRG.
出版信息
Biochem Biophys Res Commun. 1988 Dec 30;157(3):883-90. doi: 10.1016/s0006-291x(88)80957-4.
An antiserum (AS 98) was raised against a synthetic peptide deduced from published cDNA sequences of the alpha-subunit of the putative G-protein, GZ (Fong et al. Proc. Natl. Acad. Sci. USA 85, 3066-3070, 1988; Matsuoka et al. Proc. Natl. Acad. Sci. USA 85, 5384-5388, 1988). In membrane and cytosol preparations of many but not all tested mammalian tissues, AS 98 predominantly recognized two proteins of 40 and 43 kDa Mr. Whereas high levels of a 40 kDa GZ alpha-subunit were found in rat liver membranes and in brain cytosol, AS 98 failed to detect the alpha-subunit of GZ in brain membranes.
针对从推测的G蛋白GZ的α亚基已发表的cDNA序列推导出来的合成肽制备了一种抗血清（AS 98）（方等人，《美国国家科学院院刊》85，3066 - 3070，1988；松冈等人，《美国国家科学院院刊》85，5384 - 5388，1988）。在许多但并非所有测试的哺乳动物组织的膜和胞质溶胶制剂中，AS 98主要识别出两种分子量分别为40 kDa和43 kDa的蛋白质。虽然在大鼠肝膜和脑胞质溶胶中发现了高水平的40 kDa GZα亚基，但AS 98未能在脑膜中检测到GZ的α亚基。
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