基于声化学法的石墨相氮化碳包覆的氧化钒纳米复合材料用于化疗药物的灵敏电化学测定

A sensitive electrochemical determination of chemotherapy agent using graphitic carbon nitride covered vanadium oxide nanocomposite; sonochemical approach.

机构信息

Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Research and Development Center for Smart Textile Technology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.

Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan.

出版信息

Ultrason Sonochem. 2019 Nov;58:104664. doi: 10.1016/j.ultsonch.2019.104664. Epub 2019 Jun 29.

DOI:10.1016/j.ultsonch.2019.104664

PMID:31450375

Abstract

We have developed a graphitic carbon nitride covered vanadium oxide nanocomposite (VO@g-CN) by a simple sonochemical approach (50 kHz and 150 W/cm). Furthermore, the morphology and chemical composition of the VO@g-CN nanocomposite was carried out by X-rays diffractometry (XRD), transmission electron microscopy (TEM) and electrochemical impedance spectroscopy (EIS). Furthermore, the VO@g-CN nanocomposite modified electrode was investigate electrochemical behavior of the anticancer drug. Compared with bare SPCE, VO/SPCE and g-CN/SPCE, VO@g-CN modified SPCE showed highest current response towards anti-cancer drug (methotrexate). Furthermore, the modified sensor exhibits with a sharp peaks and wide linear range (0.025-273.15 μM) by using DPV with the sensitivity of 7.122 μA μM cm. Notably, we have achieved a nanomolar detection limit (13.26 nM) for the DPV detection of methotrexate. Further, the practicability of the VO@g-CN nanocomposite modified sensor can be used for real time sensing of methotrexate in drug and blood serum samples with good recover ranges. It has potential applications in routine analysis with high specificity, excellent reproducibility and good stability.

摘要

我们通过简单的超声化学方法（50 kHz 和 150 W/cm）开发了一种石墨相氮化碳覆盖的氧化钒纳米复合材料（VO@g-CN）。此外，通过 X 射线衍射（XRD）、透射电子显微镜（TEM）和电化学阻抗谱（EIS）对 VO@g-CN 纳米复合材料的形貌和化学组成进行了研究。此外，还研究了 VO@g-CN 纳米复合材料修饰电极对抗癌药物的电化学行为。与裸 SPCE、VO/SPCE 和 g-CN/SPCE 相比，VO@g-CN 修饰的 SPCE 对抗癌药物（甲氨蝶呤）表现出最高的电流响应。此外，修饰后的传感器在使用 DPV 时具有尖锐的峰和较宽的线性范围（0.025-273.15 μM），灵敏度为 7.122 μA μM cm。值得注意的是，我们实现了对甲氨蝶呤的纳摩尔检测限（13.26 nM）。此外，VO@g-CN 纳米复合材料修饰传感器的实用性可用于药物和血清样品中甲氨蝶呤的实时传感，具有良好的回收率范围。它具有常规分析的潜在应用，具有高特异性、出色的重现性和良好的稳定性。