Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China; School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China; College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, PR China.
Talanta. 2019 Dec 1;205:120126. doi: 10.1016/j.talanta.2019.120126. Epub 2019 Jul 8.
Inspired by the porous and fibrous structure of commercially available bamboo, herein we created an l-glutaminase enzyme reactor based on bamboo sticks. The enzyme was immobilized onto the bamboo sticks through a glutaraldehyde modification to achieve covalent bonding. The enzymatic hydrolysis efficiency of the prepared l-glutaminase@bamboo sticks based porous enzyme reactor was evaluated by chiral ligand exchange capillary electrochromatography using l-glutamine as the substrate. l-glutaminase@bamboo exhibited improved enzymatic hydrolysis performances, including high hydrolysis efficiency (maximum rate V: two fold higher than the free enzyme), prolonged stability (14 days) and good reusability. l-Glutaminase@bamboo sticks also expanded application capability in pharmaceutical industry in enzyme inhibitor screening. These excellent properties could be attributed to the micropores of bamboo sticks, which led to the fast enzymatic kinetics. The results suggest that the pores of bamboo sticks played an important role in the proposed enzyme reactor during the hydrolysis of l-glutamine and l-glutaminase inhibitor screening.
受市售竹材的多孔和纤维结构启发，我们在此创建了一种基于竹棒的 l-谷氨酰胺酶酶反应器。通过戊二醛修饰将酶固定在竹棒上，实现共价键合。通过手性配体交换毛细管电色谱法，以 l-谷氨酰胺为底物评估了制备的 l-谷氨酰胺酶@竹棒多孔酶反应器的酶解效率。l-谷氨酰胺酶@竹表现出改善的酶解性能，包括高效率（最大速率 V：比游离酶高两倍）、延长的稳定性（14 天）和良好的可重复使用性。l-谷氨酰胺酶@竹棒还扩展了在酶抑制剂筛选中的制药工业应用能力。这些优异的性能可归因于竹棒的微孔，这导致了快速的酶动力学。结果表明，在 l-谷氨酰胺水解和 l-谷氨酰胺酶抑制剂筛选过程中，竹棒的微孔在提出的酶反应器中发挥了重要作用。
