Department of Anatomy and Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Minerva Foundation Institute for Medical Research, Helsinki, Finland.
Nat Methods. 2019 Sep;16(9):866-869. doi: 10.1038/s41592-019-0512-x. Epub 2019 Aug 26.
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
生长素诱导降解结构域技术允许快速和受控的蛋白质消耗。然而，没有生长素的基础降解和低效的生长素诱导消耗限制了它的实用性。我们已经鉴定了一个由生长素受体 F-box 蛋白 AtAFB2 和短降解结构域 miniIAA7 组成的有效生长素诱导降解结构域系统。该系统显示出最小的基础降解，并能够在 1 小时内快速诱导内源人跨膜、细胞质和核蛋白的生长素诱导消耗，具有强大的功能表型。
