Takase M, Tsutsui K, Kawashima S
Zoological Institute, Faculty of Science, Hiroshima University, Japan.
Endocrinol Jpn. 1988 Apr;35(2):285-93. doi: 10.1507/endocrj1954.35.285.
The aim of this study is to examine the influence of Sertoli cells on LH binding to Leydig cells in culture in immature mice. Leydig cells and Sertoli cells were obtained from the testes of immature C57BL/6Ncrj mice and were cultured in serum-free medium for 7 days. The LH binding to Leydig cells and the FSH binding to Sertoli cells were dependent on incubation time, the number of cells, and the amount of labelled hormone added. The dissociation constant for LH binding to Leydig cells was 7.3 x 10(-10) M. Co-culture of Leydig cells with Sertoli cells for 7 days decreased LH binding to Leydig cells. The binding was 34.9% of that to Leydig cells cultured alone. After cultivation of Leydig cells with spent Sertoli cell-cultured medium (SM) for the last 4 days of the 7-day culture period, LH binding to Leydig cells decreased to as low as 17.4% of that of the controls. For the controls, LH binding was measured in Leydig cells cultured in spent Leydig cell-cultured medium (LM). There was no difference between SM- and LM-cultures in the final survival rate or the percentage of cells showing histochemically demonstrated 3 beta-hydroxysteroid dehydrogenase activity. These data suggest that some factor or factors are secreted from the cultured Sertoli cells and inhibit the binding of LH to Leydig cells in culture.
本研究的目的是检测支持细胞对未成熟小鼠培养的睾丸间质细胞结合促黄体生成素（LH）的影响。睾丸间质细胞和支持细胞取自未成熟C57BL/6Ncrj小鼠的睾丸，并在无血清培养基中培养7天。LH与睾丸间质细胞的结合以及促卵泡生成素（FSH）与支持细胞的结合取决于孵育时间、细胞数量和添加的标记激素量。LH与睾丸间质细胞结合的解离常数为7.3×10⁻¹⁰M。睾丸间质细胞与支持细胞共培养7天会降低LH与睾丸间质细胞的结合。其结合量为单独培养的睾丸间质细胞结合量的34.9%。在7天培养期的最后4天，用支持细胞培养后的培养基（SM）培养睾丸间质细胞，LH与睾丸间质细胞的结合降至对照组的17.4%。对于对照组，在睾丸间质细胞培养后的培养基（LM）中培养的睾丸间质细胞中测量LH结合情况。在最终存活率或显示组织化学证实的3β-羟基类固醇脱氢酶活性的细胞百分比方面，SM培养和LM培养之间没有差异。这些数据表明，培养的支持细胞分泌了某种因子或某些因子，抑制了培养中LH与睾丸间质细胞的结合。
