Rozas-Dennis Gabriela S, Marti Gerardo A, González Nadia Lorena, Guhl Felipe, Guérin Diego M A
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina.
Centro de Estudios Parasitológicos y de Vectores (CEPAVE CCT-La Plata-CONICET-UNLP), La Plata, Argentina.
J Biol Methods. 2016 May 23;3(2):e40. doi: 10.14440/jbm.2016.46. eCollection 2016.
Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of (TrV, : ) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
恰加斯病的病媒目前通过使用多种化学杀虫剂进行控制，不过人们一直在寻找针对致病病媒的替代生态方法。一种有效的方法包括使用特定病原体作为生物防治剂。这项工作的目的是描述一种针对锥蝽的完整实验接种方案。血腔接种技术可用于接种不同种类的微生物，如病毒、真菌、细菌和原生动物；因此，它可被视为感染性生物测定中的一种有用工具。本文包括对几种锥蝽物种中（TrV，: ）感染性评估的结果。该方案也适用于任何其他种类的昆虫，它描述了安全接种昆虫所需的材料和步骤，防止任何损害和/或污染。
