Research and Development Center of Biorational Pesticide , Northwest A&F University , Yangling 712100 , Shaanxi , China.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests , Hainan University , Ministry of Education, Haikou 570228 , Hainan , China.
J Agric Food Chem. 2019 Sep 18;67(37):10448-10457. doi: 10.1021/acs.jafc.9b02951. Epub 2019 Sep 9.
Carabrone is isolated from Franch. et Sav, which has good fungicidal activity, especially for (). According to previous studies, we speculated that carabrone targets the mitochondrial enzyme complex III of . To elucidate the mode of action, we used carabrone to induce oxidative stress and apoptosis in . Incubation with carabrone reduced the burst of reactive oxygen species (ROS) and mitochondrial membrane potential, as well as phosphatidylserine release. Carabrone caused ROS accumulation in mycelia by inhibiting the activity of antioxidase enzymes, among which inhibition of glutathione reductase (GR) activity was most obvious. The catalytic center of GR consists of l-cysteine residues that react with the α-methylene-γ-butyrolactone active site of carabrone. Additionally, a positive TUNEL reaction led to diffusion of the DNA electrophoresis band and upregulation of and We propose that carabrone inhibits antioxidant enzymes and promotes ROS overproduction, which causes membrane hyperpermeability, release of apoptotic factors, activation of the mitochondria-mediated apoptosis pathway, and fungal cell apoptosis.
从 Franch. et Sav 中分离得到的 Carabrone 具有良好的杀菌活性，特别是对（）。根据以往的研究，我们推测 Carabrone 的作用靶点是 的线粒体酶复合物 III。为了阐明其作用机制，我们使用 Carabrone 诱导 中的氧化应激和细胞凋亡。Carabrone 孵育降低了活性氧（ROS）和线粒体膜电位的爆发，以及磷脂酰丝氨酸的释放。Carabrone 通过抑制抗氧化酶的活性导致菌丝中 ROS 的积累，其中对谷胱甘肽还原酶（GR）活性的抑制最为明显。GR 的催化中心由 l-半胱氨酸残基组成，与 Carabrone 的α-亚甲基-γ-丁内酯活性位点反应。此外，阳性 TUNEL 反应导致 DNA 电泳带的弥散和 和 的上调。我们提出 Carabrone 抑制抗氧化酶并促进 ROS 的过度产生，导致膜通透性增加、凋亡因子释放、线粒体介导的凋亡途径激活以及真菌细胞凋亡。
