Qiu G, Gauchat J F, Wirthmüller U, De Weck A L, Stadler B M
Institute of Clinical Immunology, Inselspital, University of Bern, Switzerland.
Lymphokine Res. 1988 Winter;7(4):385-92.
Lymphokine production in human peripheral blood mononuclear cells (PBMNCs) was investigated by in situ hybridization with 35S-labelled RNA probes coding for interleukin 2 (IL-2), gamma-interferon (gamma-IFN) and granulocyte/macrophage colony stimulating factor (GM-CSF). The proportion of PBMNCs expressing lymphokine mRNA varied according to the stimuli used and followed similar patterns to those previously observed by Northern blot analysis. Only a relatively low proportion of PBMNCs expressed lymphokine mRNA after stimulation for a given time, and this seems not to be due to the heterogeneity of T cells.
通过用编码白细胞介素2（IL-2）、γ干扰素（γ-IFN）和粒细胞/巨噬细胞集落刺激因子（GM-CSF）的35S标记RNA探针进行原位杂交，研究了人外周血单个核细胞（PBMNCs）中的淋巴因子产生情况。表达淋巴因子mRNA的PBMNCs比例根据所用刺激物的不同而有所变化，并且遵循与先前通过Northern印迹分析观察到的相似模式。在给定时间的刺激后，只有相对较低比例的PBMNCs表达淋巴因子mRNA，这似乎不是由于T细胞的异质性所致。
