Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India.
Prep Biochem Biotechnol. 2020;50(1):18-27. doi: 10.1080/10826068.2019.1658117. Epub 2019 Aug 27.
We report on the development of a functionalized membrane-based technology for selective enrichment of milk fat globules from raw bovine milk. Functionalization was conducted by polymerization of acrylic acid within a polyvinylidene fluoride membrane, followed by the electrostatic attachment of a cationic polymer to impart a net positive charge. The functionalized membrane-based technology enabled a one-step method of selective separation of globules directly from milk-based on size and charge. The presence of globules in the eluate was confirmed by fluorescence microscopy. Quantification of the extracted phospholipids from globules in the eluant revealed a significantly higher amount of polar lipids than the permeate. Our study describes a comprehensive analysis of selective enrichment of fat globules using a functionalized membrane and demonstrates the beneficial effect of extracted phospholipids from enriched fat globules.
我们报告了一种基于功能化膜的技术的开发，用于从生牛乳中选择性富集乳脂肪球。功能化是通过在聚偏二氟乙烯膜内聚合丙烯酸进行的，然后通过静电附着阳离子聚合物赋予净正电荷。基于大小和电荷，基于功能化膜的技术使直接从基于牛奶的乳球进行选择性分离成为可能。通过荧光显微镜确认洗脱液中乳球的存在。从洗脱液中的乳球中提取的磷脂的定量分析表明，极性脂质的量明显高于渗透物。我们的研究描述了使用功能化膜对脂肪球进行选择性富集的全面分析，并证明了从富集的脂肪球中提取的磷脂的有益效果。
