Water Affairs Research Institute, North China University of Water Resources and Electric Power, 36 Beihuan Road, Zhengzhou 450045, China; College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing 100049, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Beijing 100101, China.
Bioresour Technol. 2019 Dec;293:122040. doi: 10.1016/j.biortech.2019.122040. Epub 2019 Aug 21.
This study evaluated the effects of the inocula from natural wetland, lab-scale and full-scale anaerobic reactors on wheat straw anaerobic digestion. Three replicate batch reactors were constructed for each inoculum to investigate the reactor performances and microbial communities. Reactors seeded with full-scale reactor samples were started up most rapidly, achieved the highest methane production, and were recognized as the higher efficient reactors. The dominance of acetoclastic methanogens, including Methanosaeta and Methanoscrina, was crucial for the higher efficient reactors, whereas hydrogenotrophic methanogens were dominant in other reactors. Genus Treponema, which could enhance the cellulose degradation and conduct homoacetogenesis, was first reported to be dominant in the bacterial communities of high efficient reactors. Inoculum sources and process conditions were suggested to be the deterministic factors in shaping the microbial communities in the higher efficient reactors. These findings contribute to the startup of new anaerobic reactors.
本研究评估了天然湿地、实验室规模和全规模厌氧反应器的接种物对小麦秸秆厌氧消化的影响。为每种接种物构建了三个重复的分批式反应器，以研究反应器性能和微生物群落。以全规模反应器样品为接种物的反应器启动最快，甲烷产量最高，被认为是高效反应器。乙酸营养型产甲烷菌（包括 Methanosaeta 和 Methanoscrina）的优势对高效反应器至关重要，而其他反应器中则以氢营养型产甲烷菌为主。能够增强纤维素降解和进行同型产乙酸作用的螺旋体属（Treponema）首次被报道在高效反应器的细菌群落中占优势。接种物来源和工艺条件被认为是塑造高效反应器中微生物群落的决定性因素。这些发现有助于新厌氧反应器的启动。
