Liu Xiao-Long, Yan Qi, Yang Yi-Lin, Hou Wen, Miao Chun-Li, Peng Ying-Chuan, Dong Shuang-Lin
Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/Department of Entomology, Nanjing Agricultural University, Nanjing 210095, China.
Institute of Entomology, Jiangxi Agricultural University, Nanchang 330045, China.
Insects. 2019 Aug 26;10(9):272. doi: 10.3390/insects10090272.
Gustatory receptors (GRs) are crucial in the peripheral coding of the non-volatile compounds in insects, and thus play important roles in multiple behaviors including feeding, mating, and oviposition. However, little research has been done on GRs in lepidopteran pests. In the current work with , an important worldwide crop's pest, a candidate fructose GR gene () was cloned in full length, and its spatial and temporal expression profiles were determined by quantitative real-time PCR (qPCR). It revealed that was highly expressed in antennae of both male and female adults, as well as in larva of first, fifth and sixth instar. Functional analyses were further conducted using the oocyte system. responded specifically to D-fructose among 12 tested sugar compounds. In addition, the behavioral assay demonstrated that both female and male moths could respond with proboscis extension behavior to D-fructose applied onto the antenna, but females showed higher sensitivity than males. The results provide an important base for further elucidation of molecular mechanisms of gustation, and a potential target for development of feeding interfering technique in .
味觉受体（GRs）在昆虫非挥发性化合物的外周编码中至关重要，因此在包括取食、交配和产卵在内的多种行为中发挥重要作用。然而，针对鳞翅目害虫的味觉受体研究较少。在当前对一种重要的世界性作物害虫[具体害虫名称未给出]的研究中，一个候选果糖味觉受体基因（[基因名称未给出]）被全长克隆，并通过定量实时PCR（qPCR）确定了其时空表达谱。结果显示，[基因名称未给出]在雌雄成虫触角以及一龄、五龄和六龄幼虫中高表达。进一步利用[卵母细胞系统名称未给出]卵母细胞系统进行功能分析。在12种测试糖类化合物中，[基因名称未给出]对D - 果糖有特异性反应。此外，行为测定表明，雌雄蛾均可对涂抹在触角上的D - 果糖做出伸喙行为反应，但雌蛾比雄蛾表现出更高的敏感性。这些结果为进一步阐明味觉分子机制提供了重要基础，并为[害虫名称未给出]取食干扰技术的开发提供了潜在靶点。
