Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Maastricht, The Netherlands.
Eli Lilly and Company, Lilly Corporate Center, Indianapolis, USA.
Int J Obes (Lond). 2020 Feb;44(2):544-547. doi: 10.1038/s41366-019-0433-x. Epub 2019 Aug 27.
Fibroblast growth factor 21 (FGF21) is an important regulator of energy metabolism. FGF21 is inactivated by fibroblast activation protein (FAP). We investigated whether FGF21 and/or FAP are secreted from human white adipose tissue of individuals with obesity by measuring total FGF21, active FGF21, and FAP concentrations in arterialized blood and venous blood draining the subcutaneous abdominal adipose tissue (scAT). Measurements were performed under fasting conditions and after a high fat meal before and after diet-induced weight loss in 16 adults with BMI 27-35 kg/m. FGF21 was not released from scAT, neither before nor after weight loss in agreement with an undetectable gene expression of FGF21 in this tissue. Although scAT showed significant gene expression of FAP, no release of FAP from the tissue could be detected. The high fat meal increased postprandial circulating FGF21 but not FAP. Circulating levels of FAP but not FGF21 were significantly reduced after weight loss. On the other hand, FAP expression in scAT was increased. In conclusion, release from scAT does not appear to contribute to circulating concentrations of FGF21 and FAP and their responses to ingestion of a high fat meal or weight loss, respectively, in individuals with obesity.
成纤维细胞生长因子 21（FGF21）是能量代谢的重要调节剂。FGF21 被成纤维细胞激活蛋白（FAP）失活。我们通过测量动脉化血液和皮下腹部脂肪组织（scAT）引流静脉血中总 FGF21、活性 FGF21 和 FAP 浓度，来研究肥胖个体的人白色脂肪组织是否分泌 FGF21 和/或 FAP。在 16 名 BMI 为 27-35kg/m 的成年人中，在空腹条件下以及在高脂肪餐后和饮食诱导的体重减轻前后进行了测量。无论在减肥前后，scAT 均未释放 FGF21，这与该组织中 FGF21 的基因表达无法检测到一致。尽管 scAT 显示出 FAP 的显著基因表达，但无法检测到 FAP 从组织中的释放。高脂肪餐后会增加餐后循环 FGF21，但不会增加 FAP。减肥后，循环 FAP 水平而非 FGF21 水平显著降低。另一方面，scAT 中的 FAP 表达增加。总之，scAT 的释放似乎不会导致肥胖个体的循环 FGF21 和 FAP 浓度及其对高脂肪餐摄入或体重减轻的反应。
