Departamento de Microbiologia, Universidade Federal de Viçosa, Av. P. H. Rolfs, Viçosa, Minas Gerais, 36570-900, Brazil.
Centro de Ciências da Natureza, Universidade Federal de São Carlos, Rod. Lauri Simões de Barros - SP 189, km 12, Buri, São Paulo, 18290-000, Brazil.
Braz J Microbiol. 2019 Oct;50(4):1083-1090. doi: 10.1007/s42770-019-00135-w. Epub 2019 Aug 27.
Bacterial biofilms are involved in various medical infections and food contamination episodes and, for this reason, it is of great importance to developing new strategies of its prevention and control. The subinhibitory concentration of nisin was determined, and its effect against Staphylococcus aureus and Staphylococcus epidermidis biofilms was evaluated. Results obtained by confocal laser microscopy demonstrated morphological changes in the architecture of the structure of biofilms. The main components (polysaccharides, proteins, and extracellular DNA (eDNA)) of the biofilm matrix were determined by spectrophotometry and showed that the formation of staphylococcal biofilms in the presence of nisin results in a less dense matrix structure with modification in its constituents. These results contribute to increase the knowledge of the composition and architecture of the extracellular matrix of biofilms of S. aureus, as well as evidence that the investigation of alternative products to assist in the control and combat of biofilms is a promising strategy.
细菌生物膜与各种医学感染和食品污染事件有关，因此，开发其预防和控制的新策略非常重要。本文测定了乳酸链球菌素的亚抑菌浓度，并评估了其对金黄色葡萄球菌和表皮葡萄球菌生物膜的影响。共聚焦激光显微镜的结果显示生物膜结构的形态发生了变化。通过分光光度法测定了生物膜基质的主要成分（多糖、蛋白质和细胞外 DNA（eDNA）），结果表明，在乳酸链球菌素存在的情况下，金黄色葡萄球菌生物膜的形成导致其组成成分发生改变，基质结构变得不那么密集。这些结果有助于增加对金黄色葡萄球菌生物膜细胞外基质组成和结构的了解，并证明研究替代产品以协助控制和对抗生物膜是一种很有前途的策略。
