UNESP - São Paulo State University, Rod. Araraquara-Jaú, Km 01, Araraquara, SP, Brazil.
Braz J Microbiol. 2019 Oct;50(4):881-886. doi: 10.1007/s42770-019-00123-0. Epub 2019 Aug 27.
Enterohemorrhagic (EHEC) and enteropathogenic Escherichia coli (EPEC) are human intestinal pathogens of clinical importance and their mechanism of pathogenicity is widely studied. However, both EHEC and EPEC poorly infect mice, whereas they do not develop important characteristics of the disease, hindering studies about mechanisms of virulence in vivo. Citrobacter rodentium exhibits high similarity of its genes with these human pathogens, including the island of pathogenicity Locus of Enterocyte Effacement (LEE). Therefore, C. rodentium becomes an alternative in vivo model for microorganisms that harbor LEE. The QseC directly regulates LEE as well as virulence mechanisms on these pathogens. Here, we report a novel surface motility in C. rodentium QseC-mediated in this non-flagellated bacterium. Moreover, we show norepinephrine and ethanolamine act as environmental signals in this movement. Hence, this study clarifies a novel role of the sensor QseC in completely unreported motility process of C. rodentium.
肠出血性（EHEC）和肠致病性大肠杆菌（EPEC）是具有临床重要性的人类肠道病原体，其致病机制已得到广泛研究。然而，EHEC 和 EPEC 对小鼠的感染能力很差，而且它们不会产生疾病的重要特征，这阻碍了体内毒力机制的研究。肠侵袭性大肠杆菌（Citrobacter rodentium）与这些人类病原体具有高度相似的基因，包括致病性肠上皮细胞消失岛（LEE）。因此，C. rodentium 成为携带 LEE 的微生物的替代体内模型。QseC 直接调节 LEE 以及这些病原体的毒力机制。在这里，我们报告了一种在非鞭毛细菌 C. rodentium 中由 QseC 介导的新型表面运动。此外，我们表明去甲肾上腺素和乙醇胺在这种运动中作为环境信号发挥作用。因此，本研究阐明了传感器 QseC 在 C. rodentium 完全未报道的运动过程中的新作用。
