Cancer Nanotechnology Research Laboratory (CNRL), Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt.
Department of Industrial Pharmacy, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt.
Nanomedicine (Lond). 2019 Sep;14(18):2461-2479. doi: 10.2217/nnm-2019-0090. Epub 2019 Aug 28.
Multicompartmental lipid-protein nanohybrids (MLPNs) were developed for combined delivery of the anticancer drugs tretinoin (TRE) and genistein (GEN) as synergistic therapy of lung cancer. The GEN-loaded lipid core was first prepared and then coated with TRE-loaded zein shell via nanoprecipitation. TRE/GEN-MLPNs demonstrated a size of 154.5 nm. ion pair formation between anionic TRE and the cationic stearyl amine improved the drug encapsulation with enhanced stability of MLPNs. TRE/GEN-coloaded MLPNs were more cytotoxic against A549 cancer cells compared with combined free GEN/TRE. , lung cancer bearing mice treated with TRE/GEN-MLPNs displayed higher apoptotic caspase activation compared with mice-treated free combined GEN/TRE. TRE/GEN-MLPNs might serve as a promising parenteral nanovehicles for lung cancer therapy.
多隔室脂质-蛋白纳米杂化物 (MLPNs) 被开发用于联合递送抗癌药物维甲酸 (TRE) 和染料木黄酮 (GEN)，作为肺癌的协同治疗方法。首先制备负载 GEN 的脂质核心，然后通过纳米沉淀在其表面包覆负载 TRE 的玉米醇溶蛋白壳。TRE/GEN-MLPNs 的粒径为 154.5nm。带负电荷的 TRE 与带正电荷的硬脂胺之间形成离子对，提高了药物包封率，并增强了 MLPNs 的稳定性。与游离 GEN/TRE 联合用药相比，TRE/GEN 共载 MLPNs 对 A549 癌细胞的细胞毒性更强。与单独使用游离 GEN/TRE 治疗的小鼠相比，用 TRE/GEN-MLPNs 治疗的肺癌荷瘤小鼠中凋亡 caspase 激活更高。TRE/GEN-MLPNs 可能成为一种有前途的肺癌治疗的注射用纳米载体。
