Delaunoy J P, Langui D, Ghandour S, Labourdette G, Sensenbrenner M
Centre de Neurochimie du CNRS, Strasbourg, France.
Int J Dev Neurosci. 1988;6(2):129-36. doi: 10.1016/0736-5748(88)90037-8.
Modifications of the morphology, the proliferation and the synthesis of carbonic anhydrase of glial cells in primary cultures maintained in defined medium have been investigated under the action of basic fibroblast growth factor. Cultures contained essentially three cell types: astrocytes which expressed glial fibrillary acidic protein, oligodendrocytes which were characterized by the presence of carbonic anhydrase and precursor cells in which these two proteins were detected by immunocytochemistry. In the presence of basic fibroblast growth factor astrocytes and oligodendrocytes underwent morphological changes, characterized by a fibrous aspect; astroglial cells acquired essentially several long processes and oligodendroglial cells formed generally two long processes. The factor increased the proliferation of these two cell types. The quantity of carbonic anhydrase per oligodendrocyte was enhanced in treated cultures. The double-stained precursor cells were present between days 7 and 11 of culture in defined medium, while in the presence of fibroblast growth factor these cells were more numerous and were still present after 14 days. The basic fibroblast growth factor stimulated the proliferation of these young glial cells and modified their morphology. But the differentiation of precursor cells towards one glial cell type appeared to be delayed.
在限定培养基中维持的原代培养物中，研究了碱性成纤维细胞生长因子作用下神经胶质细胞的形态、增殖及碳酸酐酶合成的变化。培养物中主要包含三种细胞类型：表达胶质纤维酸性蛋白的星形胶质细胞、以存在碳酸酐酶为特征的少突胶质细胞以及通过免疫细胞化学检测到这两种蛋白的前体细胞。在碱性成纤维细胞生长因子存在的情况下，星形胶质细胞和少突胶质细胞发生形态变化，其特征为呈纤维状；星形胶质细胞基本上获得了几条长突起，少突胶质细胞通常形成两条长突起。该因子增加了这两种细胞类型的增殖。在经处理的培养物中，每个少突胶质细胞的碳酸酐酶量增加。在限定培养基中培养的第7至11天之间存在双染的前体细胞，而在成纤维细胞生长因子存在的情况下，这些细胞数量更多，并且在14天后仍然存在。碱性成纤维细胞生长因子刺激了这些年轻神经胶质细胞的增殖并改变了它们的形态。但是前体细胞向一种神经胶质细胞类型的分化似乎延迟了。
