Koizumi J, Inazu A, Fujita H, Takeda M, Uno Y, Kajinami K, Mabuchi H, Takeda R
Second Department of Internal Medicine, School of Medicine, University of Kanazawa, Ishikawa, Japan.
Atherosclerosis. 1988 Nov;74(1-2):1-8. doi: 10.1016/0021-9150(88)90184-0.
The presence of apo E-containing HDL in familial hypercholesterolaemia was investigated and its removal by LDL-apheresis using a dextran sulphate cellulose column was demonstrated by measurement of the apo E/apo A-I molar ratio of serum and by nondenaturing polyacrylamide gel electrophoresis followed by immunoblotting. The molar ratios of apo E/apo A-I in the density greater than 1.063 kg/l fraction of serum obtained from two homozygous patients with familial hypercholesterolaemia were higher (0.021 and 0.030) than that from normal subjects (mean +/- SE 0.011 +/- 0.002) (P less than 0.05). Polyacrylamide gel electrophoresis and immunoblotting showed an increase in apo E-containing HDL similar to HDL2, in the plasma obtained from the homozygous patient with familial hypercholesterolaemia. The increased amounts of apo E-enriched HDL were removed from plasma by adsorption with a dextran-sulphate cellulose column. These results suggested that LDL-apheresis using the dextran-sulphate cellulose column, may cause an increase in the turnover rate of the apo E-containing HDL and thus facilitate cholesterol removal from the peripheral tissues.
研究了家族性高胆固醇血症中含载脂蛋白E的高密度脂蛋白（HDL）的存在情况，并通过测量血清中载脂蛋白E/载脂蛋白A-I摩尔比以及非变性聚丙烯酰胺凝胶电泳后免疫印迹法，证明了使用硫酸葡聚糖纤维素柱进行低密度脂蛋白吸附分离（LDL-apheresis）可去除这种HDL。从两名家族性高胆固醇血症纯合子患者获得的血清中，密度大于1.063 kg/l部分的载脂蛋白E/载脂蛋白A-I摩尔比（分别为0.021和0.030）高于正常受试者（均值±标准误为0.011±0.002）（P<0.05）。聚丙烯酰胺凝胶电泳和免疫印迹显示，在家族性高胆固醇血症纯合子患者的血浆中，类似于HDL2的含载脂蛋白E的HDL有所增加。通过用硫酸葡聚糖纤维素柱吸附，可从血浆中去除增加的富含载脂蛋白E的HDL。这些结果表明，使用硫酸葡聚糖纤维素柱进行LDL-apheresis可能会导致含载脂蛋白E的HDL周转率增加，从而促进胆固醇从外周组织中清除。
