Baek Seung Hoon, Lee Seung Woo, Kim Eun Ju, Shin Dong-Hyuk, Lee Seog-Won, Park Tae Jung
Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
Well Korea Corporation, 44 Techno 11-ro, Yuseong-gu, Daejeon 34036, Republic of Korea.
ACS Omega. 2017 Mar 16;2(3):988-993. doi: 10.1021/acsomega.6b00477. eCollection 2017 Mar 31.
Organophosphorus (OP) and triazole chemicals have been commonly used as insecticides and fungicides to protect agricultural foods from harmful insects and fungi. However, these agrichemicals sometimes remain after distribution and can cause serious health and environmental issues. Therefore, it is essential to detect OPs and triazole chemicals in agricultural products. Nowadays, many detection techniques for OPs and triazole chemicals are expensive and time-consuming and require highly trained technicians. Thus, particularly rapid, simple, and sensitive detection methods are in demand for on-site screening of agrichemicals. Gold nanoparticles (AuNPs) have been utilized for applications in analytical assays and real-time monitoring in the biosensor field because of their biocompatibility and outstanding size-dependent optical properties. In this study, we used AuNPs as a detection probe, which have a size of 17 nm in diameter, a red color, and the absorbance peak at 520 nm. When imidazole was added to AuNPs mixed with the agrichemicals, the AuNPs aggregated and their colors changed to purple, causing the appearance of a new peak at 660-670 nm, which could be measured within approximately 20 s. Moreover, we developed a novel device for multiple agrichemical detections using an AuNP-aggregation-based spectrometric detection system. This portable device is light, simple, fast, and highly sensitive as well as selective. With this system, agrichemical residues can be easily detected on the spot at a low cost and in a short reaction time.
有机磷（OP）和三唑类化学品通常用作杀虫剂和杀菌剂，以保护农产品免受有害昆虫和真菌侵害。然而，这些农用化学品有时在施用后仍有残留，会引发严重的健康和环境问题。因此，检测农产品中的有机磷和三唑类化学品至关重要。如今，许多检测有机磷和三唑类化学品的技术既昂贵又耗时，还需要训练有素的技术人员。因此，对于农用化学品的现场筛查，尤其需要快速、简便且灵敏的检测方法。金纳米颗粒（AuNPs）因其生物相容性和出色的尺寸依赖性光学特性，已被用于生物传感器领域的分析检测和实时监测。在本研究中，我们使用直径为17 nm、呈红色且在520 nm处有吸收峰的金纳米颗粒作为检测探针。当将咪唑添加到与农用化学品混合的金纳米颗粒中时，金纳米颗粒会聚集，颜色变为紫色，在660 - 670 nm处出现一个新峰，大约20秒内即可测量。此外，我们开发了一种基于金纳米颗粒聚集的光谱检测系统的新型多农残检测装置。这种便携式装置轻便、简单、快速，具有高灵敏度和选择性。利用该系统，可以低成本、在短反应时间内轻松现场检测农残。
