Tsai Chun-Yi, Chung Chin-Hsiang, Hong Jin-Long
Formosa Chemicals & Fibre Corporation, No. 1, Taisu Industrial Park, Mailiao Township, Yunlin County 63801, Taiwan.
Department of Materials and Optoelectronic Science, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
ACS Omega. 2018 Apr 24;3(4):4423-4432. doi: 10.1021/acsomega.8b00124. eCollection 2018 Apr 30.
The intensity ratio between the first (373 nm) and the third (383 nm) vibronic peaks [ / , as the pyrene (Py) scale] of fluorescent Py was used to monitor the critical concentration, drug-loading, and -releasing behaviors of a Py-terminated, amphiphilic polypeptide PPM and its hydrogen-bonded interpolymer complex (HIPC) with poly(acrylic acid) (PAA). Primarily, an amphiphilic PPM with a hydrophobic Py terminal and hydrophilic methoxy-bis(ethylene oxide) pendant groups was synthesized through multiple preparative steps, and the resultant PPM was thoroughly mixed with PAA through a preferable hydrogen bond (H bond) interaction to form HIPC. The emission study suggested that the / ratio and the quantum yield (Φ) are effective in determining the critical concentrations of the aqueous PPM and PPM/PAA solutions. Moreover, the / ratio and Φ were found to be convenient measures for determining the amounts of doxorubicin drugs loaded by and released from the aqueous PPM and PPM/PAA solutions.
荧光芘（Py）的第一（373 nm）和第三（383 nm）振动峰之间的强度比[ / ，作为芘（Py）标度]用于监测芘封端的两亲性多肽PPM及其与聚丙烯酸（PAA）形成的氢键聚合物间复合物（HIPC）的临界浓度、药物负载和释放行为。首先，通过多个制备步骤合成了具有疏水芘末端和亲水甲氧基 - 双（环氧乙烷）侧基的两亲性PPM，然后通过优选的氢键（H键）相互作用将所得的PPM与PAA充分混合以形成HIPC。发射研究表明， / 比值和量子产率（Φ）可有效确定PPM水溶液和PPM/PAA溶液的临界浓度。此外，发现 / 比值和Φ是确定PPM水溶液和PPM/PAA溶液负载和释放的阿霉素药物量的便捷方法。
