Research Unit Bioactive Natural Products and Biotechnology, Faculty of Pharmacy of Monastir, University of Monastir, Monastir, Tunisia.
Department of Biochemistry, General Medicine Faculty of Monastir, Monastir, Tunisia.
Nutr Cancer. 2020;72(5):747-756. doi: 10.1080/01635581.2019.1654528. Epub 2019 Aug 28.
A large number of plant-derived products have been approved for the treatment of numerous types of cancer, and these products have also shown to reduce the effects of metastatic cancer. The aim of this study is to evaluate the anticancer effects of a methanolic extract of root (M extract) against B16F10 melanoma cancer cells as well as . It was shown to induce apoptosis, , and to inhibit cell growth by arresting cell cycle progression in SubG1 phase. Mice bearing the melanoma cells were used to confirm any effectiveness of the M extract as an antitumor promoting agent. In mice dosed with 50 mg M/kg/d (for 28 days), tumor weight was inhibited by 65.03% compared to that in mice that did not receive the product. Our results demonstrate on the one hand, that this inhibition was accompanied by a drastic decrease regulation of complex FAK, Src, ERK, p130Cas and paxillin. On the other hand, it was marked by a measurable decrease of the metastatic descent in the lungs. These effects could be ascribed to the presence of bryoniosides and cucurbitacins such as cucurbitacin A and cucurbitacin G in M extract.
大量植物源性产品已被批准用于治疗多种类型的癌症，并且这些产品已被证明可以减轻转移性癌症的影响。本研究旨在评估根的甲醇提取物（M 提取物）对 B16F10 黑色素瘤癌细胞的抗癌作用，以及。结果表明，它通过将细胞周期进程阻滞在 SubG1 期来诱导细胞凋亡、细胞周期停滞和抑制细胞生长。使用携带黑色素瘤细胞的小鼠来确认 M 提取物作为抗肿瘤促进剂的任何效果。在接受 50mg M/kg/d（28 天）剂量的小鼠中，与未接受该产品的小鼠相比，肿瘤重量抑制了 65.03%。我们的结果一方面表明，这种抑制伴随着 FAK、Src、ERK、p130Cas 和桩蛋白的复杂调节的急剧下降。另一方面，它标志着肺部转移下降可测量。这些作用可能归因于 M 提取物中存在的 Bryoniosides 和葫芦素，如葫芦素 A 和葫芦素 G。
