Laboratory Research on Biological Systems and Geomatics, Faculty of Nature and Life, University of Mascara, Algeria.
J Ethnopharmacol. 2012 May 7;141(1):510-6. doi: 10.1016/j.jep.2012.02.052. Epub 2012 Mar 21.
Bryonia dioica Jacq. is a climbing perennial herb with tuberous roots which is widely used in traditional medicine in Algeria for the treatment of cancers; it belongs to the genus Bryonia (Cucurbitaceae).
To investigate the cytotoxic and apoptogenic activities, the phytochemical composition and acute toxicity of the aqueous extract of Bryonia dioica roots growing in Algeria.
Dried roots of Bryonia dioica were extracted with water (decoction). The cytotoxic effects of the aqueous extract in the Burkitt's lymphoma BL41 cell lines were evaluated by flow cytometry. Apoptosis induction was assessed by two corroborative assays; propidium iodide (PI) staining of cell DNA and flow cytometric light scatter analysis. The mitochondria membrane potential was investigated using a fluorescent dye DIOC6. The expression of caspases-3, -8, -9 and PARP was assessed by Western blot. The phytochemical screening of the roots of Bryonia dioica was performed using qualitative phytochemical standard procedures.
The Bryonia dioica aqueous extract induced cell death in a dose-dependent manner. The IC50 of Bryonia dioica aqueous extract was estimated to be approximately 15, 63μg/ml. This was accompanied by induction of apoptosis, activation of caspase-3 and -9, cleavage of PARP and loss of mitochondria membrane potential. Furthermore, the phytochemical screening of roots of Bryonia dioica showed the presence of various bioactive such as polyphenols, sterols and triterpenes, alkaloids, c-heterosides, carbohydrates and saponins.
The aqueous extract of Bryonia dioica induces apoptosis in the Burkitt's lymphoma BL41 cell lines via the mitochondrial pathway. The flavonoids, sterols and triterpens detected could be responsible for the cytotoxic and apoptogenic activities of the aqueous extract of Bryonia dioica. These findings suggest that Bryonia dioica could be considered as a promising source for developing novel therapeutics against Burkitt's lymphoma.
黑牵牛（Bryonia dioica Jacq.）是一种攀援多年生草本植物，具有块根，在阿尔及利亚传统医学中广泛用于治疗癌症；它属于黑牵牛属（葫芦科）。
研究阿尔及利亚生长的黑牵牛根的水提物的细胞毒性和促凋亡活性、植物化学成分和急性毒性。
黑牵牛干根用水（煎剂）提取。通过流式细胞术评估水提取物在伯基特淋巴瘤 BL41 细胞系中的细胞毒性作用。通过两种相互证实的检测方法评估细胞凋亡诱导；碘化丙啶（PI）染色细胞 DNA 和流式细胞术光散射分析。使用荧光染料 DIOC6 研究线粒体膜电位。通过 Western blot 评估半胱天冬酶-3、-8、-9 和 PARP 的表达。使用定性植物化学标准程序对黑牵牛根进行植物化学筛选。
黑牵牛水提取物以剂量依赖的方式诱导细胞死亡。黑牵牛水提取物的 IC50 约为 15、63μg/ml。这伴随着细胞凋亡的诱导、半胱天冬酶-3 和 -9 的激活、PARP 的切割和线粒体膜电位的丧失。此外，黑牵牛根的植物化学筛选表明存在各种生物活性物质，如多酚、甾醇和三萜、生物碱、C-糖苷、碳水化合物和皂苷。
黑牵牛水提取物通过线粒体途径诱导伯基特淋巴瘤 BL41 细胞系凋亡。检测到的类黄酮、甾醇和三萜可能是黑牵牛水提取物细胞毒性和促凋亡活性的原因。这些发现表明，黑牵牛可以被认为是开发针对伯基特淋巴瘤的新型治疗方法的有前途的来源。
