Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, Cascavel, Paraná, Brazil.
J Appl Microbiol. 2019 Dec;127(6):1706-1715. doi: 10.1111/jam.14426. Epub 2019 Oct 8.
The aim of this work was to characterize and apply a polygalacturonase of Penicillium janthinellum new strain VI2R3M.
The polygalacturonase obtained from P. janthinellum VI2R3M was incubated in cultures of passion fruit peel and was partially purified by ion-exchange chromatography and gel filtration. The enzyme showed a relative molecular mass of 102·0 kDa, maximum activity at pH 5·0, temperature of 50°C, 100% stablity at 50°C and 80% stablity at pH 3·0-5·0. The apparent K , V and K values for hydrolyzing polygalacturonic acid were 2·56 mg ml , 163·1 U mg and 277 s respectively. The polygalacturonase presented exo activity and was activated by Mg . The juices treated with polygalacturonase presented increases in transmittance with reduction in colour.
The results suggest that the new lineage P. janthinellum VI2R3M presents a high yield of an exo-polygalacturonase induced by agro-industrial residues, with excellent activity and stability in acidic pH and at 50°C.
The use of agro-industrial residue to obtain the polygalacturonase can contribute to a decrease enzyme production cost. The results of the activity, stability to acidic pH and excellent performance in the clarification of juices show that the enzyme is promising for industrial application.
本工作旨在对一株新的青霉属菌株 VI2R3M 的聚半乳糖醛酸酶进行特性描述并加以应用。
从青霉属 VI2R3M 获得的聚半乳糖醛酸酶在西番莲果皮培养物中进行孵育，并通过离子交换色谱和凝胶过滤进行部分纯化。该酶的相对分子质量为 102.0 kDa，最适 pH 值为 5.0，最适温度为 50°C，在 50°C 下具有 100%的稳定性，在 pH 3.0-5.0 下具有 80%的稳定性。水解聚半乳糖醛酸的表观 K 、 V 和 K 值分别为 2.56 mg ml 、 163.1 U mg 和 277 s。该聚半乳糖醛酸酶具有外切酶活性，可被 Mg 激活。经聚半乳糖醛酸酶处理的果汁透光率增加，颜色变浅。
结果表明，新青霉属 VI2R3M 株系在农业工业废料的诱导下可产生高产量的外切聚半乳糖醛酸酶，在酸性 pH 和 50°C 下具有极好的活性和稳定性。
利用农业工业废料获得聚半乳糖醛酸酶可降低酶的生产成本。该酶在酸性 pH 下的活性、稳定性以及澄清果汁的出色性能表明，该酶具有广阔的工业应用前景。
