Ogilvie L M, Ramsden D B
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, U.K.
Toxicol Lett. 1988 Dec;44(3):281-7. doi: 10.1016/0378-4274(88)90167-1.
Ioxynil, an iodine-containing compound with structural similarity to T3 affects the thyroids of experimental animals. Binding to human plasma proteins was compared with that of T3, to gain insight into possible effects in humans. After incubating human serum with [125I]ioxynil, 2DIEP against anti-whole human serum and rocket immunoelectrophoresis against anti-TBPA, albumin or TBG were carried out. Ioxynil bound to TBPA but not to TBG nor albumin. The Ka calculated for the TBPA-ioxynil complex was 2.6 x 10(6) l.mol-1. Ioxynil competed for the iodothyronine-binding sites of TBPA.
碘苯腈是一种与三碘甲状腺原氨酸（T3）结构相似的含碘化合物，会影响实验动物的甲状腺。将其与人血浆蛋白的结合情况与T3进行比较，以深入了解其对人类可能产生的影响。用[125I]碘苯腈与人血清孵育后，进行了针对抗全人血清的二维免疫电泳以及针对抗甲状腺结合前白蛋白（TBPA）、白蛋白或甲状腺素结合球蛋白（TBG）的火箭免疫电泳。碘苯腈与TBPA结合，但不与TBG和白蛋白结合。计算得出的TBPA - 碘苯腈复合物的解离常数（Ka）为2.6×10(6) l·mol-1。碘苯腈竞争TBPA的碘甲状腺原氨酸结合位点。
