Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.
Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Comp Biochem Physiol B Biochem Mol Biol. 2019 Nov;237:110328. doi: 10.1016/j.cbpb.2019.110328. Epub 2019 Aug 25.
Insulin-like growth factors (Igf1 and Igf2) play a key role in growth and development of vertebrates. In mammals, the expression of IGFs is regulated by estradiol-17β (E) via estrogen receptors (ESRs). The expression of igfs can also be regulated by E in fish, while comparative study of this is still lacking. The present study examined tissue distribution of igfs and hepatic expression of igfs and esrs during gonad development in Scatophagus argus by real-time PCR. Serum E concentration was measured by enzyme-linked immunosorbent assay (ELISA). The hepatic expression of igfs and esrs at gonadal phase III, incubated with either E (0.1, 1 or 10 μM) alone or in combination with estrogen receptor antagonists-fulvestrant, MPP or PHTPP, was measured. igf1 and igf2 expressed highest in liver of both sexes. Igf1, esr1 and esr2b expressions and serum E concentration increased, while igf2 and esr2a expressions decreased, during ovary development. Igfs and esrs expressions increased while serum E concentration maintained low during testis development. In females, E incubation enhanced the expressions of igf1 and esr1 but inhibited that of igf2 and esr2a. Both fulvestrant and MPP inhibited up-regulation effect of E on igf1 and esr1. Fulvestrant enhanced down-regulation effect of E on igf2 and esr2a, but MPP conversely. In males, E incubation enhanced the expressions of igfs, esr1 and esr2a. Fulvestrant and MPP inhibited up-regulation effect of E on igfs and esr1. PHTPP inhibited igf1 and esr2 expressions in both sexes. Our results indicated that the expression of igfs is regulated by E via Esrs in S. argus.
胰岛素样生长因子（Igf1 和 Igf2）在脊椎动物的生长和发育中起着关键作用。在哺乳动物中，IGFs 的表达受雌激素受体（ESRs）介导的雌二醇-17β（E）调节。鱼类中的 igfs 表达也可以被 E 调节，而对此的比较研究仍然缺乏。本研究通过实时 PCR 检测了 Scatophagus argus 性腺发育过程中 igfs 的组织分布和肝组织中 igfs 和 esrs 的表达，并通过酶联免疫吸附测定（ELISA）测量了血清 E 浓度。在单独或与雌激素受体拮抗剂氟维司群、MPP 或 PHTPP 联合孵育时，测量了性腺期 III 期的肝组织中 igfs 和 esrs 的表达。igf1 和 igf2 在两性肝脏中表达最高。在卵巢发育过程中，igf1、esr1 和 esr2b 的表达和血清 E 浓度增加，而 igf2 和 esr2a 的表达减少。在睾丸发育过程中，igfs 和 esrs 的表达增加，而血清 E 浓度保持较低水平。在雌性中，E 孵育增强了 igf1 和 esr1 的表达，但抑制了 igf2 和 esr2a 的表达。氟维司群和 MPP 均抑制了 E 对 igf1 和 esr1 的上调作用。氟维司群增强了 E 对 igf2 和 esr2a 的下调作用，但 MPP 则相反。在雄性中，E 孵育增强了 igfs、esr1 和 esr2a 的表达。氟维司群和 MPP 抑制了 E 对 igfs 和 esr1 的上调作用。PHTPP 抑制了两性的 igf1 和 esr2 的表达。我们的结果表明，在 S. argus 中，igfs 的表达受 E 通过 Esrs 调节。
