Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Fishery Eco-Environment Monitoring Center of Lower Reaches of Yangtze River, Ministry of Agriculture/Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Environmental Factors (Wuxi), Ministry of Agriculture, No. 9 Shanshui East Rd., Wuxi, Jiangsu, 214081, People's Republic of China.
Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing, 100141, People's Republic of China.
Arch Microbiol. 2020 Jan;202(1):43-53. doi: 10.1007/s00203-019-01712-1. Epub 2019 Aug 28.
To evaluate the changes in bacterial diversity at various time points under resveratrol supplementation, we aimed to investigate the diversification of gut microbiota and the changes in total genetic diversity. We performed 16S rDNA gene sequencing at different time points (15, 30, and 45 days) to analyze the gut microbiota of tilapia. Fusobacteria, Proteobacteria, and Bacteroidetes (15 days) or Cyanobacteria (30 and 45 days) were found to be the three most abundant phyla. Cyanobacteria (15 and 30 days), Proteobacteria (15 days), Firmicutes and Chlamydiae (30 and 45 days), Planctomycetes (30 days), Bacteroidetes, Actinobacteria, and Fusobacteria (45 days) in the 0.05 g/kg RES group increased as compared to that in the controls. Proteobacteria and Cyanobacteria significantly decreased and increased at 30 and 45 days, respectively, while the reverse pattern was observed at 15 days. The Bacteroidetes:Firmicutes and Proteobacteria:Cyanobacteria ratios were significantly increased (15 and 45 days, P < 0.05) and decreased (30 days, P < 0.05). RES supplementation did not affect the richness and diversity of the gut microbiota in tilapia. Our findings may contribute to the development of strategies for the management of diseases.
为了评估白藜芦醇补充后不同时间点细菌多样性的变化，我们旨在研究肠道微生物群的多样化和总遗传多样性的变化。我们在不同时间点（15、30 和 45 天）进行了 16S rDNA 基因测序，以分析罗非鱼的肠道微生物群。厚壁菌门、变形菌门和拟杆菌门（15 天）或蓝细菌门（30 和 45 天）是最丰富的三个门。与对照组相比，0.05g/kg RES 组中的蓝细菌门（15 和 30 天）、变形菌门（15 天）、Firmicutes 和 Chlamydiae（30 和 45 天）、浮霉菌门（30 天）、拟杆菌门、放线菌门和厚壁菌门（45 天）增加。变形菌门和蓝细菌门在 30 天和 45 天分别显著减少和增加，而在 15 天则呈现相反的模式。Bacteroidetes:Firmicutes 和 Proteobacteria:Cyanobacteria 的比例显著增加（15 和 45 天，P<0.05）和减少（30 天，P<0.05）。RES 补充剂对罗非鱼肠道微生物群的丰富度和多样性没有影响。我们的发现可能有助于制定疾病管理策略。
