Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Fishery Eco-Evironment Monitoring Center of Lower Reaches of Yangtze River, Ministry of Agriculture/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture, Wuxi, Jiangsu, 214081, PR China.
Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu, 214081, PR China.
Fish Shellfish Immunol. 2018 Jun;77:200-207. doi: 10.1016/j.fsi.2018.03.040. Epub 2018 Mar 21.
The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is cultured widely for production of freshwater fish in China, while streptococcosis, likely related to pathogenic infections, occurs frequently in juvenile, mother, and operated GIFT. The gut microbiota plays an important role in nutrient digestibility in animals, and resveratrol (RES) has been used in feed for different freshwater fish species. Therefore, understanding changes in the tilapia gut microbiota across different concentrations of dietary RES supplementation is extremely important. The gut microbiota population in tilapia at 45 d after supplementation with different concentrations (0, 0.025, 0.05, 0.1 g/kg) of dietary RES was assessed by 16S rDNA gene sequencing. A total of 5445 operational taxonomic units were identified from all samples, and 14 phyla and 81 families were identified from all fecal samples. The bacteria of the phylum Firmicutes were significantly enriched in the 0.025 g/kg RES group when compared with the controls. Proteobacteria, Firmicutes and Cyanobacteria were the most dominant three phyla in all samples. With the increasing concentrations, the proportion of beneficial microbial taxa (Acetobacteraceae and Methylobacteriaceae) increased, whereas the proportion of harmful microbial taxa decreased, eg. Streptococcaceae except for 0.1 g/kg RES groups. RES did not affect the richness and diversity in tilapia gut microbiota. These findings provide information on the diversity and differences in GIFT gut microbiota database, and may contribute to developing strategies for management of diseases and long-term sustainability of O. niloticus culture.
基因改良的养殖罗非鱼（GIFT，Oreochromis niloticus）在中国广泛用于淡水鱼养殖，但链球菌病可能与致病性感染有关，经常发生在幼年、母鱼和手术 GIFT 中。肠道微生物群在动物的营养消化中起着重要作用，白藜芦醇（RES）已被用于不同淡水鱼类的饲料。因此，了解不同浓度膳食 RES 补充剂对罗非鱼肠道微生物群的影响非常重要。通过 16S rDNA 基因测序评估了补充不同浓度（0、0.025、0.05、0.1g/kg）膳食 RES 后 45 天罗非鱼肠道微生物群的种群。从所有样品中鉴定出 5445 个操作分类单元，从所有粪便样品中鉴定出 14 个门和 81 个科。与对照组相比，添加 0.025g/kg RES 组的厚壁菌门细菌明显富集。变形菌门、厚壁菌门和蓝藻门是所有样品中最主要的三个门。随着浓度的增加，有益微生物类群（醋杆菌科和甲基杆菌科）的比例增加，而有害微生物类群（除 0.1g/kg RES 组外的链球菌科）的比例减少。RES 对罗非鱼肠道微生物群的丰富度和多样性没有影响。这些发现提供了 GIFT 肠道微生物群数据库多样性和差异的信息，并可能有助于制定疾病管理和奥尼罗非鱼养殖长期可持续性的策略。
