Treadwell E L, Swanson M S, Byrd A W
Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354.
Diagn Clin Immunol. 1988;5(6):361-6.
Ribonucleoprotein (RNP) antigen-antibody precipitin bands obtained by two-dimensional double immunodiffusion were systematically analyzed and compared using a microcomputer and a SPSS-PC statistical analysis package. Precipitins rated in respective agarose concentrations ranging from 0.3% to 1.5% were obtained from twofold dilutions of a calf thymus nuclear extract (CTNE) containing RNP antigen. These dilutions were reacted against the same dilutions of plasma containing anti-RNP antibody. A Mean Quality Index Score was calculated at each agarose concentration from the sum of ratings assigned for the intensity (thickness) of precipitin bands times the sum of ratings assigned for the clarity (clearness and sharpness) of the precipitin bands. This product was divided by the total number of possible precipitin reactions. The Mean Quality Index Score for the 0.4% agarose was significantly higher than the other concentrations (p less than or equal to 0.05). This method or similar approaches may allow a more systematic way of comparing precipitins developed in gel immunodiffusion.
利用微型计算机和SPSS-PC统计分析软件包，对通过二维双向免疫扩散获得的核糖核蛋白（RNP）抗原-抗体沉淀带进行了系统分析和比较。从含有RNP抗原的小牛胸腺核提取物（CTNE）的两倍稀释液中，在琼脂糖浓度范围为0.3%至1.5%的情况下获得沉淀素。这些稀释液与含有抗RNP抗体的血浆的相同稀释液反应。在每个琼脂糖浓度下，根据沉淀带强度（厚度）的评级总和乘以沉淀带清晰度（清晰度和锐度）的评级总和来计算平均质量指数得分。该乘积除以可能的沉淀反应总数。0.4%琼脂糖的平均质量指数得分显著高于其他浓度（p小于或等于0.05）。这种方法或类似方法可能会提供一种更系统的方式来比较凝胶免疫扩散中形成的沉淀素。
