Hellman L, Steen M L, Sundvall M, Pettersson U
Department of Medical Genetics, University of Uppsala, Sweden.
Gene. 1988 Aug 15;68(1):93-100. doi: 10.1016/0378-1119(88)90602-6.
The nucleotide sequences of the introns that are located between the C4 exon and the first membrane exon of mouse and rat immunoglobulin epsilon-chain genes have been determined. The rat intron sequence was found to contain four separate clusters of repetitive sequences all of which consisted of (dC-dA)n.(dG-dT)n dinucleotide repeats. A comparison between this chromosomal region in mouse and rat revealed four deletions or duplications, three of which have occurred inside or at the borders of the CA clusters. Rearrangements have occurred inside or at the borders of all four repeats after the evolutionary separation of mouse and rat. The sequence comparison reveals in addition a duplication, connected to the CA repeats, which has occurred early in evolution, before the evolutionary divergence of mouse and rat. These findings suggest that (dC-dA)n.(dG-dT)n sequences are potential targets for recombination events.
已确定小鼠和大鼠免疫球蛋白ε链基因中位于C4外显子与首个膜外显子之间的内含子的核苷酸序列。发现大鼠内含子序列包含四个独立的重复序列簇，所有这些簇均由(dC-dA)n.(dG-dT)n二核苷酸重复序列组成。对小鼠和大鼠的该染色体区域进行比较发现了四处缺失或重复，其中三处发生在CA簇内部或边界处。在小鼠和大鼠进化分离后，所有四个重复序列内部或边界处均发生了重排。序列比较还揭示了与CA重复序列相关的一次重复，该重复发生在进化早期，即小鼠和大鼠进化分歧之前。这些发现表明，(dC-dA)n.(dG-dT)n序列是重组事件的潜在靶点。
