通过 CRISPR 敲除宫颈癌 miR-214 对基因表达的转录组分析。
Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells.
机构信息
Cancer Biology Lab, School of Biotechnology, KIIT University, Bhubaneswar, India.
Bionivid Technology Pvt. Ltd., Kasturi Nagar, Bangalore, India.
出版信息
Genomics. 2020 Mar;112(2):1490-1499. doi: 10.1016/j.ygeno.2019.08.020. Epub 2019 Aug 26.
In this study, we investigate the effect of one such micro RNA, miR-214 which is frequently down-regulated in cervical cancer. In this study, we either CRISPR knocked out or overexpressed miR-214 in cervical cancer cells and analyzed the global mRNA expression by Next Generation Sequencing (NGS) It was observed that a total of 108 genes were upregulated and 178 downregulated between the samples, above and below the baseline respectively. Gene Ontology and KEGG pathway analysis reveal distinct biological processes and pathways. Analysis of gene regulatory networks also gave different network patterns in the two samples. We confirmed the RNA sequencing data for 10 genes; IFIF27, SMAD3, COX11, TP53INP1, ABL2, FGF8, TNFAIP3, NRG1, SP3 and MDM4 by Real-time PCR. This is the first report on the effect of miR-214 on global mRNA profile in cervical cancer cells. This study also reports new biomarkers for cervical cancer prognosis.
在这项研究中，我们研究了一种这样的 micro RNA，即 miR-214，它在宫颈癌中经常下调。在这项研究中，我们通过下一代测序（NGS）对宫颈癌细胞中的 miR-214 进行了 CRISPR 敲除或过表达，并分析了全局 mRNA 表达。结果观察到，在基线以上和以下的样本之间，共有 108 个基因上调，178 个基因下调。基因本体论和 KEGG 通路分析揭示了不同的生物学过程和通路。基因调控网络的分析也给出了两个样本中不同的网络模式。我们通过实时 PCR 对 10 个基因（IFIF27、SMAD3、COX11、TP53INP1、ABL2、FGF8、TNFAIP3、NRG1、SP3 和 MDM4）的 RNA 测序数据进行了验证。这是首次报道 miR-214 对宫颈癌细胞全局 mRNA 谱的影响。本研究还报告了宫颈癌预后的新生物标志物。
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