College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.
Infect Genet Evol. 2019 Nov;75:104017. doi: 10.1016/j.meegid.2019.104017. Epub 2019 Aug 26.
Hepatitis-hydropericardium syndrome (HHS) is an acute infectious disease caused by fowl adenovirus serotype 4 (FAdV-4), which mainly infects broilers aged 3-5 weeks. In March 2018, a pathogenic disease, which was characterized by symptoms similar to HHS, broke out in 10-day-old broiler flocks in Shandong province. In this study, a strain of FAdV-4 (SDSG) was isolated from naturally infected broilers. To assess its pathogenicity, 10-day-old and 20-day-old specific pathogen-free (SPF) chickens were inoculated separately with the FAdV-4 virus fluid via oral and intramuscular injection routes. The results show that typical hydropericardium and hepatitis were observed in experimental chickens. The titer levels of the virus antibody and the levels of inflammatory cytokines were upregulated, which may be caused by the infection and innate immune response. The detection of viral load showed that the presence of virus was detected in multiple organs, in which the liver contained the highest concentration of viral DNA, and the virus content in the intramuscular injection group was higher than that of the oral injection group. In summary, these findings increase our understanding of the pathogenicity of FAdV-4 (SDSG) in chickens. The established model will be valuable for anti-viral drug testing and vaccine evaluation, which can prevent and reduce the spread of HHS in the poultry industry.
肝炎-心包积水综合征（Hepatitis-hydropericardium syndrome，HHS）是由禽腺病毒血清型 4（Fowl adenovirus serotype 4，FAdV-4）引起的一种急性传染病，主要感染 3-5 周龄的肉鸡。2018 年 3 月，山东省 10 日龄肉鸡群暴发了一种以 HHS 为特征的致病性疾病。本研究从自然感染的肉鸡中分离到一株 FAdV-4（SDSG）。为评估其致病性，分别通过口服和肌肉注射途径用 FAdV-4 病毒液对 10 日龄和 20 日龄 SPF 鸡进行接种。结果显示，实验鸡出现典型的心包积液和肝炎。病毒抗体滴度和炎症细胞因子水平上调，这可能是由感染和固有免疫反应引起的。病毒载量检测表明，在多个器官中检测到病毒存在，其中肝脏中含有最高浓度的病毒 DNA，肌肉注射组的病毒含量高于口服注射组。综上所述，这些发现增加了我们对 FAdV-4（SDSG）在鸡中的致病性的认识。建立的模型将对抗病毒药物检测和疫苗评价具有重要价值，可预防和减少家禽业中 HHS 的传播。
