Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, PR China.
Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2019 Nov;237:110324. doi: 10.1016/j.cbpb.2019.110324. Epub 2019 Aug 26.
The ATF/CREB family of transcription factors represents a large group of basic region-leucine zipper (bZip) proteins that regulate diverse cellular responses. Here we carried out a comprehensive analysis of ATF/CREB family members in 22 representative animal species. The family probably originated from the early diverging metazoan and significantly expanded in vertebrates due to multiple whole genome duplication. Duplicates of atf6 were derived from 2R, and duplicates of creb1, crem, jdp2, creb5, atf4, atf5 and atf7 were products of 3R. We also isolated 21 ATF/CREBs, belonging to 6 subfamilies from Nile tilapia. Based on transcriptome data, most members were found to be dominantly expressed in the head kidney, heart, brain and testis. Some ATF/CREBs displayed sexual dimorphic expression in gonad at 5, 90 and 180 dah (days after hatching), but not at 30 dah. creb1a and atf4a were found to be expressed mainly in phase I and II oocytes of the ovary; while creb1b and atf4b mainly in spermatogenic cells of the testis, indicating divergence of duplicated genes from 3R which suggested neofunctionalization or subfunctionalization in gonad. This is the first genome-wide screening and evolutionary analysis of ATF/CREB family in different animals, particularly in teleosts. The expression analysis of this family in tilapia gonad provided a fundamental clue for understanding their important roles in sex differentiation and gonadal development in teleosts.
ATF/CREB 家族转录因子是一大类碱性亮氨酸拉链 (bZip) 蛋白，它们调节多种细胞反应。在这里，我们对 22 种代表性动物物种中的 ATF/CREB 家族成员进行了全面分析。该家族可能起源于早期分化的后生动物，并由于多次全基因组复制而在脊椎动物中显著扩张。atf6 的重复序列来自 2R，creb1、crem、jdp2、creb5、atf4、atf5 和 atf7 的重复序列则是 3R 的产物。我们还从尼罗罗非鱼中分离出 21 种 ATF/CREB，属于 6 个亚家族。基于转录组数据，大多数成员在头肾、心脏、大脑和睾丸中表达丰富。一些 ATF/CREB 在性腺中表现出性别二态性表达，在 5、90 和 180 天（孵化后），但在 30 天没有。creb1a 和 atf4a 主要在卵巢的 I 期和 II 期卵母细胞中表达；而 creb1b 和 atf4b 主要在睾丸的精子发生细胞中表达，表明 3R 复制的基因发生了趋异，这表明在性腺中发生了新功能化或亚功能化。这是对不同动物，特别是硬骨鱼类中 ATF/CREB 家族的首次全基因组筛选和进化分析。该家族在罗非鱼性腺中的表达分析为理解它们在硬骨鱼类性别分化和性腺发育中的重要作用提供了基本线索。
