硫酸盐还原菌（SRB）和非硫酸盐还原菌（soNRB）的微生物分带增强了微好氧条件下 SR-DSR 工艺的性能。
The microbial zonation of SRB and soNRB enhanced the performance of SR-DSR process under the micro-aerobic condition.
机构信息
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, HeiLongjiang Province 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, HeiLongjiang Province 150090, China.
出版信息
Environ Int. 2019 Nov;132:105096. doi: 10.1016/j.envint.2019.105096. Epub 2019 Aug 26.
The micro-aerobic condition has proven to effectively enhance the COD removal and elemental sulfur (S) transformation rate in the sulfate reduction-denitrifying sulfide removal (SR-DSR) process. However, the mechanisms of how micro-aerobic condition enhances S transformation remain largely unknown. Therefore in this work an integrated investigation was performed to document the mechanisms and the effect of different startup modes (micro-aerobic startup (termed as mSR-DSR) and anaerobic startup (termed as aSR-DSR)) on bioreactor performance and microbial community dynamics. The results showed that micro-aerobic startup achieved a shorter period to reach a stable performance for SR-DSR, which could be one of the factors affecting the choice of the bioreactor startup mode considering engineering application. For all the tested conditions, removal of nitrate, sulfate and lactate were 100%, >80% and 100%, respectively. The maximum transformation rate of elemental sulfur in mSR-DSR was 57%, which was higher than that in aSR-DSR. The mechanism explorations revealed that micro-aerobic condition not only particularly enriched the sulfide-oxidizing, nitrate-reducing bacteria (soNRB) but also promoted the microbial zonation of sulfate-reducing bacteria (SRB) and soNRB, thereby achieving more S transformation in the effluent. Under micro-aerobic condition, SRB were mainly distributed in the bottom and middle part of the reactor, while soNRB were assembled in the top. The relative abundance of soNRB in both aSR-DSR and mSR-DSR notably increased to 41.5% and 23.7% at the top when 5 mL air min L was applied. Furthermore, the degradation of organic carbon was also accelerated under micro-aerobic condition, possibly due to the enrichment of organic compounds degrading bacteria Bacteroidetes_vadin HA17.
微氧条件已被证明能有效地提高硫酸盐还原-反硝化硫化物去除（SR-DSR）过程中的 COD 去除率和元素硫（S）转化速率。然而，微氧条件增强 S 转化的机制在很大程度上仍然未知。因此，在这项工作中，进行了综合研究，以记录不同启动模式（微氧启动（称为 mSR-DSR）和厌氧启动（称为 aSR-DSR））对生物反应器性能和微生物群落动态的影响。结果表明，微氧启动能更快地达到 SR-DSR 的稳定性能，这可能是考虑工程应用时选择生物反应器启动模式的因素之一。在所有测试条件下，硝酸盐、硫酸盐和乳酸的去除率均为 100%、>80%和 100%。mSR-DSR 中元素硫的最大转化速率为 57%，高于 aSR-DSR。机制探索表明，微氧条件不仅特别富集了硫氧化、硝酸盐还原菌（soNRB），而且促进了硫酸盐还原菌（SRB）和 soNRB 的微生物分区，从而在出水处实现更多的 S 转化。在微氧条件下，SRB 主要分布在反应器的底部和中部，而 soNRB 则组装在顶部。当施加 5 mL air min L 时，soNRB 在 aSR-DSR 和 mSR-DSR 中的相对丰度分别显著增加到顶部的 41.5%和 23.7%。此外，在微氧条件下，有机碳的降解也得到了加速，这可能是由于有机化合物降解菌拟杆菌属（Bacteroidetes_vadin HA17）的富集。
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