School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
Core Research Laboratory Clinical Research Institute Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, South Korea.
Adv Exp Med Biol. 2019;1155:3-11. doi: 10.1007/978-981-13-8023-5_1.
Taurine content in an older brain is decreased compared to a younger brain and is associated with cognitive deficits. It is not yet known whether the decrease in taurine content is associated with decreased expression of taurine inflow mediating transporters during the aging process. In this study, we investigated whether aging affects taurine transporter and glycine transporter 1 expression in the brain cortex of the mouse. Taurine and glycine transporter expression was compared in the brain cortex of C57BL/6 mice at different ages (2, 12, and 24 months) and to age-matched NLRP3 inflammasome knockout mice. In wild type mice, taurine transporter (TauT) expression in the brain cortex of 12- or 24-month-old mice did not significantly differ from TauT expression in 2-month-old mice. Moreover, TauT expression in the brain cortex of 12- or 24-month-old mice did not significantly differ from age-matched NLRP3 KO mice. This result indirectly suggests that TauT expression may be not affected by aging or age-induced inflammation. In addition, glycine transporter expression was similar to the TauT expression pattern. In conclusion, aging and age-related inflammation might not significantly affect taurine and glycine transporter expression in aged mice. Thus, the decrease of taurine content in an older brain, which is associated with cognitive deficits, may not be significantly related to altered taurine and glycine transporter expression.
与年轻大脑相比，老年人大脑中的牛磺酸含量降低，并且与认知缺陷有关。目前尚不清楚牛磺酸含量的降低是否与衰老过程中牛磺酸流入介导转运体的表达减少有关。在这项研究中，我们研究了衰老是否会影响小鼠大脑皮层中的牛磺酸转运体和甘氨酸转运体 1 的表达。比较了不同年龄（2、12 和 24 个月）的 C57BL/6 小鼠和年龄匹配的 NLRP3 炎症小体敲除小鼠大脑皮层中的牛磺酸和甘氨酸转运体表达。在野生型小鼠中，12 或 24 个月大的小鼠大脑皮层中的牛磺酸转运体（TauT）表达与 2 个月大的小鼠的 TauT 表达无显著差异。此外，12 或 24 个月大的小鼠大脑皮层中的 TauT 表达与年龄匹配的 NLRP3 KO 小鼠无显著差异。这一结果间接表明 TauT 表达可能不受衰老或衰老引起的炎症的影响。此外，甘氨酸转运体的表达与 TauT 表达模式相似。总之，衰老和与年龄相关的炎症可能不会显著影响老年小鼠中牛磺酸和甘氨酸转运体的表达。因此，与认知缺陷相关的老年人大脑中牛磺酸含量的降低可能与牛磺酸和甘氨酸转运体表达的改变没有显著关系。
