乙醇和牛磺酸单独及联合修饰糖尿病大鼠肾功能指标的变化。

Modification by Ethanol and Taurine, Singly and in Combination, of Changes in Indices of Renal Dysfunction Caused by Diabetes in Rats.

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy and Health Professions, St. John's University, Jamaica, NY, USA.

出版信息

Adv Exp Med Biol. 2019;1155:369-380. doi: 10.1007/978-981-13-8023-5_35.

DOI:10.1007/978-981-13-8023-5_35

PMID:31468415

Abstract

The present study was carried out in diabetic rats to examine the effects of ethanol (EtOH) and taurine (TAU), singly and in combination, in reducing the changes of laboratory test values indicating renal dysfunction. For this purpose, male Sprague-Dawley rats, 250-280 g in weight and in groups of 6, were made diabetic with a single, 60 mg/kg intraperitoneal dose of streptozotocin in 10 mM citrate buffer pH 4.5. On day 15 and for the remaining 14 days of the study, the diabetic rats (a) started to drink 5% EtOH in place of water, (b) received a single daily 2.4 mM/kg oral dose of TAU or (c) were allowed to drink 5% EtOH after receiving a dose of TAU. Starting from day 28 and ending on day 29, a 24 h urine sample was collected, its volume was measured, and then used to measure glucose (GLC), total protein (TP) and electrolytes (Na, K, Ca, Mg). Blood samples collected immediately thereafter via cardiac puncture were processed for the plasma fractions which were analyzed for their creatinine (CRT) and urea nitrogen (UN) contents. In comparison to normal (control) rats, diabetic ones showed a higher output of urine (+5.6-fold), a massive increase in plasma GLC (+473%), passed more GLC (+73.8-fold) and TP (+8.2-fold) in the urine, showed higher plasma CRT (+241%) and UN (+74%) levels, a lower plasma UN/CRT ratio (-47%) and a greater output of electrolytes in the urine (by at least twofold). By themselves both EtOH and TAU were found to markedly lower the effects of diabetes, with EtOH generally appearing more effective than TAU. However, the concurrent availability of EtOH and TAU was found to be more protective than either treatment alone.

摘要

本研究旨在观察乙醇（EtOH）和牛磺酸（TAU）单独和联合使用对减少糖尿病大鼠实验室检查值肾功能改变的影响。为此，雄性 Sprague-Dawley 大鼠，体重 250-280g，6 只一组，腹腔内注射 60mg/kg 的链脲佐菌素（10mM 柠檬酸盐缓冲液，pH4.5）制成糖尿病模型。第 15 天和研究的剩余 14 天，糖尿病大鼠（a）开始饮用 5% EtOH 替代水，（b）接受每日 2.4mM/kg 的 TAU 单次口服剂量，或（c）饮用 5% EtOH 后接受 TAU 剂量。从第 28 天开始到第 29 天结束，收集 24 小时尿液样本，测量其体积，然后用于测量葡萄糖（GLC）、总蛋白（TP）和电解质（Na、K、Ca、Mg）。随后立即通过心脏穿刺采集血液样本，处理血浆部分，分析其肌酐（CRT）和尿素氮（UN）含量。与正常（对照）大鼠相比，糖尿病大鼠的尿液排泄量增加（增加 5.6 倍），血浆 GLC 大量增加（增加 473%），尿液中 GLC（增加 73.8 倍）和 TP（增加 8.2 倍）增加，血浆 CRT（增加 241%）和 UN（增加 74%）水平升高，血浆 UN/CRT 比值降低（降低 47%），尿液中电解质排泄量增加（至少增加两倍）。乙醇（EtOH）和牛磺酸（TAU）本身都能显著降低糖尿病的影响，乙醇的作用通常比 TAU 更明显。然而，同时使用乙醇（EtOH）和牛磺酸（TAU）比单独使用任何一种治疗方法更具保护作用。